Department of Bioengineering, University of California, Berkeley , Berkeley, California 94720, United States.
Biological Systems and Engineering, Lawrence Berkeley National Laboratory , Berkeley, California 94720 United States.
Biomacromolecules. 2016 Jul 11;17(7):2409-16. doi: 10.1021/acs.biomac.6b00515. Epub 2016 Jun 10.
We developed rubber-like elastomeric materials using a natural elastin derived sequence and genetic engineering to create precisely defined elastin-like polypeptides. The coiled elastin-like polypeptide chains, which behave like entropic springs, were cross-linked using an end-to-end tethering scheme to synthesize simple hydrogels with excellent extensibility and reversibility. Our hydrogels extend to strains as high as 1500% and remain highly resilient with elastic recovery as high as 94% even at 600% strain, significantly exceeding any other protein-based hydrogel. These materials are valuable as elastomeric hydrogels for designing extremely robust scaffolds useful for tissue engineering.
我们使用天然弹性蛋白衍生序列和基因工程开发了类似橡胶的弹性体材料，以精确设计弹性蛋白样多肽。这些卷曲的弹性蛋白样多肽链表现得像熵弹簧，通过端到端的连接方案交联，合成具有优异可扩展性和可恢复性的简单水凝胶。我们的水凝胶可延伸至高达 1500%的应变，即使在 600%的应变下，弹性回复率仍高达 94%，远远超过任何其他基于蛋白质的水凝胶。这些材料作为弹性水凝胶具有很高的价值，可用于设计用于组织工程的极其坚固的支架。
