Marston Chung K, Ibrahim Hisham, Lee Philip, Churchwell George, Gumke Megan, Stanek Danielle, Gee Jay E, Boyer Anne E, Gallegos-Candela Maribel, Barr John R, Li Han, Boulay Darbi, Cronin Li, Quinn Conrad P, Hoffmaster Alex R
National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.
Villages Regional Hospital, Lady Lake, FL, United States of America.
PLoS One. 2016 Jun 3;11(6):e0156987. doi: 10.1371/journal.pone.0156987. eCollection 2016.
Bacillus cereus isolates have been described harboring Bacillus anthracis toxin genes, most notably B. cereus G9241, and capable of causing severe and fatal pneumonias. This report describes the characterization of a B. cereus isolate, BcFL2013, associated with a naturally occurring cutaneous lesion resembling an anthrax eschar. Similar to G9241, BcFL2013 is positive for the B. anthracis pXO1 toxin genes, has a multi-locus sequence type of 78, and a pagA sequence type of 9. Whole genome sequencing confirms the similarity to G9241. In addition to the chromosome having an average nucleotide identity of 99.98% when compared to G9241, BcFL2013 harbors three plasmids with varying homology to the G9241 plasmids (pBCXO1, pBC210 and pBFH_1). This is also the first report to include serologic testing of patient specimens associated with this type of B. cereus infection which resulted in the detection of anthrax lethal factor toxemia, a quantifiable serum antibody response to protective antigen (PA), and lethal toxin neutralization activity.
蜡样芽孢杆菌分离株已被描述为携带炭疽芽孢杆菌毒素基因，最显著的是蜡样芽孢杆菌G9241，并且能够引起严重的致命性肺炎。本报告描述了一株蜡样芽孢杆菌分离株BcFL2013的特征，该分离株与一个类似炭疽焦痂的自然发生的皮肤损伤有关。与G9241相似，BcFL2013对炭疽芽孢杆菌pXO1毒素基因呈阳性，多位点序列类型为78，pagA序列类型为9。全基因组测序证实了其与G9241的相似性。除了与G9241相比染色体平均核苷酸同一性为99.98%外，BcFL2013还携带三个与G9241质粒（pBCXO1、pBC210和pBFH_1）具有不同同源性的质粒。这也是第一份包括对与这种蜡样芽孢杆菌感染相关的患者标本进行血清学检测的报告，该检测导致检测到炭疽致死因子毒血症、对保护性抗原（PA）的可量化血清抗体反应以及致死毒素中和活性。
