Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Environ Toxicol Pharmacol. 2016 Jul;45:28-40. doi: 10.1016/j.etap.2016.05.010. Epub 2016 May 13.
4-Vinylcyclohexene diepoxide (VCD) is an industrial occupational health hazard chemical because it induces ovotoxicity in rodents. The current study investigated the impacts of VCD on selected hepatic and renal markers of oxidative stress and inflammation in both sexes of Wistar rats. Thus, male and female rats were randomly distributed into four groups of ten rats per group, and dosed orally with VCD for 28days. The control male and female groups of rats received corn oil only, while each of the three remaining groups of both sexes of rats received VCD (100, 250 and 500mg/kg BW) respectively. Thereafter, biomarkers of hepatic and renal oxidative damage, inflammation and immunohistochemical expressions of iNOS, COX-2, caspase-9 and caspase-3 were evaluated. The results revealed that VCD increased markers of liver and kidney functions, oxidative damage and inflammation, and disrupted the antioxidant homeostasis of the rats (p<0.05). Lastly, VCD enhanced the immunohistochemical expressions of iNOS, COX-2, caspase-9 and caspase-3 in the liver of the rats. Thus, our data imply that VCD induced toxicity in the liver and kidney of rats via the combined impacts of oxidative damage and inflammation.
4-乙烯基环己烯二氧化物（VCD）是一种工业职业健康危害化学物质，因为它会导致啮齿动物的卵毒性。本研究调查了 VCD 对雄性和雌性 Wistar 大鼠的某些肝和肾氧化应激和炎症标志物的影响。因此，雄性和雌性大鼠被随机分为四组，每组 10 只大鼠，并口服给予 VCD 28 天。雄性和雌性对照组大鼠仅给予玉米油，而其余三组大鼠分别给予 VCD（100、250 和 500mg/kg BW）。然后，评估了肝和肾氧化损伤、炎症的生物标志物以及 iNOS、COX-2、caspase-9 和 caspase-3 的免疫组化表达。结果表明，VCD 增加了肝和肾功能、氧化损伤和炎症的标志物，并破坏了大鼠的抗氧化平衡（p<0.05）。最后，VCD 增强了大鼠肝脏中 iNOS、COX-2、caspase-9 和 caspase-3 的免疫组化表达。因此，我们的数据表明，VCD 通过氧化损伤和炎症的综合影响诱导了大鼠的肝和肾毒性。
