sesC作为一种遗传标记，用于从其他分离株中轻松鉴定表皮葡萄球菌。
sesC as a genetic marker for easy identification of Staphylococcus epidermidis from other isolates.
作者信息
Khodaparast Ladan, Khodaparast Laleh, Van Mellaert Lieve, Shahrooei Mohammad, Van Ranst Marc, Van Eldere Johan
机构信息
KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, Department of Cellular and Molecular Medicine, Switch Laboratory, Herestraat 49, box 802, B-3000 Leuven, Belgium.
KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, Department of Cellular and Molecular Medicine, Switch Laboratory, Herestraat 49, box 802, B-3000 Leuven, Belgium.
出版信息
Infect Genet Evol. 2016 Sep;43:222-4. doi: 10.1016/j.meegid.2016.05.037. Epub 2016 May 31.
Staphylococcus epidermidis is one of the major concerns with respect to hospital-acquired infections. Therefore, a rapid and easy method to identify at species level S. epidermidis isolates out of a broad range of bacteria is necessary. Based on earlier studies, the sesC gene encoding a S. epidermidis surface protein revealed to be a highly conserved gene in this species. By means of an easy and inexpensive PCR assay, the presence of sesC was checked in 438 clinical staphylococcal isolates. Results showed that sesC is specifically present in all S. epidermidis. In conclusion, the sesC gene can be exploited as a genetic marker in order to distinguish S. epidermidis from other isolates.
表皮葡萄球菌是医院获得性感染的主要关注点之一。因此，需要一种快速简便的方法，从多种细菌中在种水平上鉴定表皮葡萄球菌分离株。基于早期研究，编码表皮葡萄球菌表面蛋白的sesC基因在该物种中是一个高度保守的基因。通过一种简单且廉价的聚合酶链反应（PCR）检测方法，对438株临床葡萄球菌分离株进行了sesC基因存在情况的检测。结果表明，sesC基因仅在所有表皮葡萄球菌中特异性存在。总之，sesC基因可作为一种遗传标记，用于将表皮葡萄球菌与其他分离株区分开来。
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