用于伊斯法罕分离株中β-内酰胺耐药性的简易鉴定、分子及表型研究的（Sesc）识别
Recognition of (Sesc) for Easy Identification of and Molecular and Phenotypic Study of Β-Lactam Resistance in Isolates in Isfahan.
作者信息
Behshood Parisa, Tajbakhsh Elahe, Momtaz Hassan
机构信息
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
出版信息
Rep Biochem Mol Biol. 2020 Oct;9(3):309-314. doi: 10.29252/rbmb.9.3.309.
BACKGROUND
Not only is it crucial to rapidly detect isolates from a broad range of bacteria, but recognizing resistance agents can greatly improve current diagnostic and therapeutic strategies.
METHODS
The current cross-sectional study investigated 120 clinical isolates from a nosocomial infection. The isolates were identified using common biochemical tests, and specific surface protein C () primers were used to confirm the presence of . PCR and special primers were used to detect the β-lactamase gene (). Methicillin resistance was measured using the agar screening method and antibiotic susceptibility was measured by disk diffusion.
RESULTS
100 samples were characterized as using a phenotypic and genotypic methods. From the 100 specimens examined, 80% contained . According to agar screening, 60% of isolates were methicillin-resistant. isolates demonstrated the highest resistance to penicillin (93%) and the highest sensitivity to cefazolin (39%).
CONCLUSION
The increased resistance to β-lactam antibiotics in isolates is alarming, and certain precautions should be taken by healthcare systems to continuously monitor the antimicrobial pattern of , so that an appropriate drug treatment can be established.
背景
快速检测多种细菌的分离株至关重要，而且识别耐药因子能够极大地改善当前的诊断和治疗策略。
方法
当前的横断面研究调查了120株来自医院感染的临床分离株。使用常规生化试验鉴定分离株，并使用特异性表面蛋白C（）引物确认的存在。采用聚合酶链反应（PCR）和特异性引物检测β-内酰胺酶基因（）。采用琼脂筛选法测定耐甲氧西林情况，采用纸片扩散法测定抗生素敏感性。
结果
采用表型和基因型方法将100个样本鉴定为。在所检测的100个标本中，80%含有。根据琼脂筛选结果，60%的分离株对甲氧西林耐药。分离株对青霉素的耐药性最高（93%），对头孢唑林的敏感性最高（39%）。
结论
分离株对β-内酰胺类抗生素耐药性的增加令人担忧，医疗系统应采取某些预防措施，持续监测的抗菌模式，以便制定适当的药物治疗方案。
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