Cui Rui, Lu Qingjun, Teng Yufei, Li Kun, Li Na
a Bejing Tongren Eye Center, Beijing Key Laboratory of Ophthalmology And Visual Sciences, Beijing Tongren Hospital, Capital Medical University , Beijing , China.
b Informatic Center, China-Japan friendship hospital , Beijing , China.
Curr Eye Res. 2017 Jan;42(1):21-27. doi: 10.3109/02713683.2016.1145235. Epub 2016 Jun 3.
To investigate the mechanism of chitosan promoting corneal wound healing though evaluating its effect on extracellular signal-regulated kinases (ERK) and p38 pathway activity in a rabbit animal model.
Cell proliferation and migration assay were performed 24 hours after chitosan treatment. The activity of ERK and p38 pathways was detected by using immunofluorescence and Western blotting in the presence of chitosan and an ERK inhibitor. In vivo study of epithelial debridement wounds was performed on 8 mm rabbit corneas in the presence of chitosan and an ERK pathway inhibitor.
Immunostaining with Ki67 and migrating assay showed that chitosan could upregulate the cell proliferation and promote the cell migration. Chitosan activated the ERK pathway in 5 min to 30 min after treatment but did not affect the p38 pathway. ERK inhibitor PD98059 can inhibit the chitosan-stimulated ERK phosphorylation. Chitosan increased the corneal epithelial wound closure in organ culture and ERK inhibition with PD98059 blocked the effect of chitosan on wound healing.
Chitosan promoted corneal epithelial proliferation and migration during the wound healing in rabbits' eye. Chitosan-stimulated epithelial wound healing is partially mediated through the activation of the ERK pathway but not the p38 pathway.
通过评估壳聚糖对兔动物模型中细胞外信号调节激酶（ERK）和p38信号通路活性的影响，研究壳聚糖促进角膜伤口愈合的机制。
壳聚糖处理24小时后进行细胞增殖和迁移试验。在壳聚糖和ERK抑制剂存在的情况下，使用免疫荧光和蛋白质印迹法检测ERK和p38信号通路的活性。在壳聚糖和ERK信号通路抑制剂存在的情况下，对8mm兔角膜进行上皮清创伤口的体内研究。
Ki67免疫染色和迁移试验表明，壳聚糖可上调细胞增殖并促进细胞迁移。壳聚糖在处理后5分钟至30分钟激活ERK信号通路，但不影响p38信号通路。ERK抑制剂PD98059可抑制壳聚糖刺激的ERK磷酸化。壳聚糖增加了器官培养中角膜上皮伤口的闭合，而用PD98059抑制ERK则阻断了壳聚糖对伤口愈合的作用。
壳聚糖促进兔眼角膜上皮在伤口愈合过程中的增殖和迁移。壳聚糖刺激的上皮伤口愈合部分是通过激活ERK信号通路而非p38信号通路介导的。
