Fischak Corinna, Klaus Robert, Werkmeister René M, Hohenadl Christine, Prinz Martin, Schmetterer Leopold, Garhöfer Gerhard
Center for Medical Physics and Biomedical Engineering, Medical University Vienna, Vienna, Austria.
Christian Doppler Laboratory for Ocular Effects of Thiomers, Vienna, Austria.
J Ophthalmol. 2017;2017:5192924. doi: 10.1155/2017/5192924. Epub 2017 Jun 12.
The present study was performed to investigate the effect of topically administered chitosan--acetylcysteine (C-NAC) on corneal wound healing in a rabbit model.
A total of 20 New Zealand White rabbits were included in the randomized, masked, placebo-controlled experiment. A monocular epithelial debridement was induced by manual scraping under general anesthesia. Animals were randomized to receive either C-NAC two times daily or placebo. Monitoring of corneal wound healing was performed with ultra-high-resolution optical coherence tomography (OCT) and epithelial fluorescein staining. Measurements were done immediately after and up to 72 hours after wound induction.
No difference in wound size was found immediately after surgical debridement between the C-NAC group and the placebo group. Wound healing was significantly faster in the C-NAC group compared to the placebo group ( < 0.01 for both methods). A good correlation was found between the OCT technique and the epithelial fluorescein staining in terms of wound size ( = 0.94).
Administration of C-NAC containing eye drops twice daily leads to a faster corneal wound healing in a rabbit model of corneal debridement as compared to placebo. Ultra-high-resolution OCT is considered a noninvasive, dye-free alternative to conventional fluorescein staining in assessing corneal wound healing also in humans.
本研究旨在探讨局部应用壳聚糖 - 乙酰半胱氨酸（C-NAC）对兔角膜伤口愈合的影响。
20只新西兰白兔纳入随机、双盲、安慰剂对照实验。在全身麻醉下通过手动刮擦诱导单眼上皮清创。动物被随机分为每日两次接受C-NAC或安慰剂。用超高分辨率光学相干断层扫描（OCT）和上皮荧光素染色监测角膜伤口愈合。在伤口诱导后立即及长达72小时进行测量。
C-NAC组和安慰剂组在手术清创后立即伤口大小无差异。与安慰剂组相比，C-NAC组伤口愈合明显更快（两种方法均P<0.01）。在伤口大小方面，OCT技术与上皮荧光素染色之间存在良好相关性（r = 0.94）。
与安慰剂相比，在兔角膜清创模型中，每日两次给予含C-NAC的滴眼液可使角膜伤口愈合更快。超高分辨率OCT被认为是评估人类角膜伤口愈合时一种无创、无需染料的传统荧光素染色替代方法。
