Zhang De-Chao, Liu Yan-Xia, Huang Hai-Jun, Weber Karin, Margesin Rosa
Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, PR China.
Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
Int J Syst Evol Microbiol. 2016 Sep;66(9):3400-3405. doi: 10.1099/ijsem.0.001208. Epub 2016 Jun 3.
A Gram-stain-negative, rod-shaped bacterial strain, designated S9-10T, was isolated from a sediment sample from the Yellow Sea near China. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain S9-10T was a member of the family Flavobacteriaceaeand formed a distinct lineage. The genomic DNA G+C content of strain S9-10T was 34.2 mol%. The major respiratory quinone was MK-6. The predominant cellular fatty acids were iso-C15 : 0 (21.1 %), iso-C15 : 1G (16.3 %) and iso-C17 : 0 3-OH (12.0 %). The polar lipid profile contained phosphatidylethanolamine, aminophospholipid, aminoglycolipid, two unidentified aminolipids and five unidentified polar lipids. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain S9-10T represents a novel species of a novel genus of the family Flavobacteriaceae, for which the name Oceanihabitans sediminis gen. nov., sp. nov. is proposed. The type strain of Oceanihabitans sediminis is S9-10T (=DSM 28133T =LMG 28074T).
从中国附近黄海的沉积物样本中分离出一株革兰氏阴性杆状细菌菌株，命名为S9-10T。16S rRNA基因序列的系统发育分析表明，菌株S9-10T是黄杆菌科的成员，并形成了一个独特的谱系。菌株S9-10T的基因组DNA G+C含量为34.2 mol%。主要呼吸醌为MK-6。主要的细胞脂肪酸为异-C15 : 0（21.1 %）、异-C15 : 1G（16.3 %）和异-C17 : 0 3-OH（12.0 %）。极性脂质谱包含磷脂酰乙醇胺、氨基磷脂、氨基糖脂、两种未鉴定的氨基脂质和五种未鉴定的极性脂质。基于表型、基因型、化学分类和系统发育分析，菌株S9-10T代表黄杆菌科一个新属的一个新物种，为此提出新属名Oceanihabitans sediminis gen. nov.，新种名Oceanihabitans sediminis sp. nov.。Oceanihabitans sediminis的模式菌株为S9-10T（=DSM 28133T =LMG 28074T）。
