van Oorschot Roland A H, Verdon Timothy J, Ballantyne Kaye N
Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod, Melbourne, VIC, 3085, Australia.
Department of Genetics, La Trobe Institute for Molecular Sciences, La Trobe University, Bundoora, Melbourne, VIC, 3083, Australia.
Methods Mol Biol. 2016;1420:1-12. doi: 10.1007/978-1-4939-3597-0_1.
Effective sampling of biological material is critical to the ability to acquire DNA profiles of probative value. The main methods of collection are swabbing, tapelifting, or direct excision. This chapter describes the key aspects to consider when applying these methods, in addition to suggested procedures for swabbing and tapelifting. Important issues to be considered, such as exhibit triaging, pre-examination preparation, contamination risk reduction, sample localization, sample identification, and sample prioritization as well as aspects of record keeping, packaging, and storage, are also raised.
有效采集生物材料对于获取具有证明价值的DNA图谱的能力至关重要。主要的采集方法有擦拭、胶带粘贴或直接切除。本章除了介绍擦拭和胶带粘贴的建议程序外，还描述了应用这些方法时需要考虑的关键方面。还提出了一些需要考虑的重要问题，如证物分类、检验前准备、降低污染风险、样本定位、样本识别和样本优先级，以及记录保存、包装和储存等方面。
