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通过犬类粪便DNA检测进行犯罪现场分析——病例报告

Crime Scene Analysis Through DNA Testing of Canine Feces-A Case Report.

作者信息

Somnay Vishal, Duong Thomas, Tsao Ray-Young, Prahlow Joseph A

出版信息

Acad Forensic Pathol. 2020 Mar;10(1):56-61. doi: 10.1177/1925362120944743. Epub 2020 Sep 14.

DOI:10.1177/1925362120944743
PMID:32983294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7495676/
Abstract

Forensic DNA testing can play a critical role in homicide investigations. Selecting the appropriate evidence on which to perform DNA testing requires foresight and reasoning based on experience and science. Although successful DNA testing can occur using many substrates, including blood, hair, and sweat/epithelial cells, positive results can also result from testing various unorthodox samples. The authors report on a triple-murder investigation where DNA testing of dog feces at the crime scene matched DNA testing of feces found on the shoe of a suspect resulting in successful prosecution of the case.

摘要

法医DNA检测在凶杀案调查中可发挥关键作用。选择合适的证据进行DNA检测需要基于经验和科学的远见与推理。尽管使用包括血液、毛发和汗液/上皮细胞在内的多种底物都能成功进行DNA检测,但检测各种非传统样本也可能得到阳性结果。作者报告了一起三重谋杀案的调查,在该案件中,对犯罪现场狗粪的DNA检测与在一名嫌疑人鞋子上发现的粪便的DNA检测相匹配,从而成功起诉了该案件。

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1
Crime Scene Analysis Through DNA Testing of Canine Feces-A Case Report.通过犬类粪便DNA检测进行犯罪现场分析——病例报告
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DNA profile of dog feces as evidence to solve a homicide.狗粪便的DNA图谱作为解决一起凶杀案的证据。
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Use of fecal material to associate a suspect with a crime scene: report of two cases.利用粪便物质将嫌疑人与犯罪现场关联起来:两起案例报告。
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本文引用的文献

1
DNA testing in homicide investigations.杀人案件调查中的DNA检测
Med Sci Law. 2017 Oct;57(4):179-191. doi: 10.1177/0025802417721790. Epub 2017 Aug 4.
2
Collection of Samples for DNA Analysis.用于DNA分析的样本采集。
Methods Mol Biol. 2016;1420:1-12. doi: 10.1007/978-1-4939-3597-0_1.
3
Leading-edge forensic DNA analyses and the necessity of including crime scene investigators, police officers and technicians in a DNA elimination database.前沿法医DNA分析以及将犯罪现场调查人员、警察和技术人员纳入DNA消除数据库的必要性。
Forensic Sci Int Genet. 2015 Nov;19:50-55. doi: 10.1016/j.fsigen.2015.06.002. Epub 2015 Jun 12.
4
Utility of stool sample-based tests for the diagnosis of Helicobacter pylori infection in children.基于粪便样本检测在儿童幽门螺杆菌感染诊断中的应用。
J Pediatr Gastroenterol Nutr. 2011 Jun;52(6):718-28. doi: 10.1097/MPG.0b013e3182077d33.
5
Analysis of fecal DNA methylation to detect gastrointestinal neoplasia.粪便DNA甲基化分析用于检测胃肠道肿瘤。
J Natl Cancer Inst. 2009 Sep 16;101(18):1244-58. doi: 10.1093/jnci/djp265. Epub 2009 Aug 21.
6
Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces.粪便采集、常温保存以及从人类粪便中提取DNA以进行细菌和人类标志物的PCR扩增。
J Microbiol Methods. 2008 Feb;72(2):124-32. doi: 10.1016/j.mimet.2007.11.007. Epub 2007 Nov 21.
7
A widely applicable protocol for DNA isolation from fecal samples.一种广泛适用的从粪便样本中分离DNA的方法。
Biochem Genet. 2006 Dec;44(11-12):503-12. doi: 10.1007/s10528-006-9050-1.
8
STR-typing of human DNA from human fecal matter using the QIAGEN QIAamp stool mini kit.使用QIAGEN QIAamp粪便微型试剂盒对人类粪便中的人类DNA进行STR分型。
J Forensic Sci. 2005 Jul;50(4):802-8.