Grant Clare F J, Carr B Veronica, Singanallur Nagendrakumar B, Morris Jacqueline, Gubbins Simon, Hudelet Pascal, Ilott Martin, Charreyre Catherine, Vosloo Wilna, Charleston Bryan
The Pirbright Institute, Ash Road, Woking, UK.
CSIRO, 5 Portarlington Road, Geelong, VIC 3220, Australia.
J Gen Virol. 2016 Sep;97(9):2201-2209. doi: 10.1099/jgv.0.000517. Epub 2016 Jun 2.
Antibodies play a pivotal role against viral infection, and maintenance of protection is dependent on plasma and memory B-cells. Understanding antigen-specific B-cell responses in cattle is essential to inform future vaccine design. We have previously defined T-cell-dependent and -independent B-cell responses in cattle, as a prelude to investigating foot-and-mouth-disease-virus (FMDV)-specific B-cell responses. In this study, we have used an FMDV O-serotype vaccination (O1-Manisa or O SKR) and live-virus challenge (FMDV O SKR) to investigate the homologous and heterologous B-cell response in cattle following both vaccination and live-virus challenge. The FMDV O-serotype vaccines were able to induce a cross-reactive plasma-cell response, specific for both O1-Manisa and O SKR, post-vaccination. Post-FMDV O SKR live-virus challenge, the heterologous O1-Manisa vaccination provided cross-protection against O SKR challenge and cross-reactive O SKR-specific plasma cells were induced. However, vaccination and live-virus challenge were not able to induce a detectable FMDV O-serotype-specific memory B-cell response in any of the cattle. The aim of new FMDV vaccines should be to induce memory responses and increased duration of immunity in cattle.
抗体在抵抗病毒感染中起关键作用，而保护作用的维持依赖于浆细胞和记忆B细胞。了解牛体内抗原特异性B细胞反应对于指导未来疫苗设计至关重要。我们之前已确定牛体内T细胞依赖性和非依赖性B细胞反应，以此作为研究口蹄疫病毒（FMDV）特异性B细胞反应的前奏。在本研究中，我们使用FMDV O型疫苗接种（O1-马尼萨或O SKR）和活病毒攻击（FMDV O SKR）来研究牛在接种疫苗和活病毒攻击后同源和异源B细胞反应。FMDV O型疫苗接种后能够诱导针对O1-马尼萨和O SKR的交叉反应性浆细胞反应。在FMDV O SKR活病毒攻击后，异源的O1-马尼萨疫苗接种提供了针对O SKR攻击的交叉保护，并诱导产生了交叉反应性的O SKR特异性浆细胞。然而，接种疫苗和活病毒攻击均未能在任何一头牛中诱导出可检测到的FMDV O型特异性记忆B细胞反应。新型FMDV疫苗的目标应该是在牛体内诱导记忆反应并延长免疫持续时间。
