Mar Cho Cho, Fan Yong, Li Fu-Li, Hu Guang-Rong
a Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Engineering Laboratory of Single Cell Oil, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
b Chemical Technology Research Center, Department of Research and Innovation, Ministry of Science and Technology , Yangon , Republic of the Union of Myanmar.
Int J Phytoremediation. 2016 Dec;18(12):1195-201. doi: 10.1080/15226514.2016.1193466.
Edible oil industry produced massive wastewater, which requires extensive treatment to remove pungent smell, high phosphate, carbon oxygen demand (COD), and metal ions prior to discharge. Traditional anaerobic and aerobic digestion could mainly reduce COD of the wastewater from oil refinery factories (WEORF). In this study, a robust oleaginous microalga Desmodesmus sp. S1 was adapted to grow in WEORF. The biomass and lipid content of Desmodesmus sp. S1 cultivated in the WEORF supplemented with sodium nitrate were 5.62 g·L(-1) and 14.49%, whereas those in the WEORF without adding nitrate were 2.98 g·L(-1) and 21.95%. More than 82% of the COD and 53% of total phosphorous were removed by Desmodesmus sp. S1. In addition, metal ions, including ferric, aluminum, manganese and zinc were also diminished significantly in the WEORF after microalgal growth, and pungent smell vanished as well. In comparison with the cells grown in BG-11 medium, the cilia-like bulges and wrinkles on the cell surface of Desmodesmus sp. S1 grown in WEORF became out of order, and more polyunsaturated fatty acids were detected due to stress derived from the wastewater. The study suggests that growing microalgae in WEORF can be applied for the dual roles of nutrient removal and biofuel feedstock production.
食用油行业产生了大量废水，在排放前需要进行广泛处理以去除刺鼻气味、高磷酸盐、化学需氧量（COD）和金属离子。传统的厌氧和好氧消化主要可以降低炼油厂废水（WEORF）的COD。在本研究中，一种健壮的产油微藻 Desmodesmus sp. S1 被驯化在WEORF中生长。在添加硝酸钠的WEORF中培养的 Desmodesmus sp. S1 的生物量和脂质含量分别为5.62 g·L(-1) 和14.49%，而在不添加硝酸盐的WEORF中培养的生物量和脂质含量分别为2.98 g·L(-1) 和21.95%。Desmodesmus sp. S1 去除了超过82%的COD和53%的总磷。此外，微藻生长后，WEORF中的铁、铝、锰和锌等金属离子也显著减少，刺鼻气味也消失了。与在BG-11培养基中生长的细胞相比，在WEORF中生长的 Desmodesmus sp. S1 的细胞表面的纤毛状凸起和皱纹变得紊乱，并且由于废水产生的压力检测到更多的多不饱和脂肪酸。该研究表明，在WEORF中培养微藻可用于营养物去除和生物燃料原料生产的双重作用。
