印度猪痘病毒的分离及基因特征分析

Isolation and genetic characterization of swinepox virus from pigs in India.

作者信息

Riyesh Thachamvally, Barua Sanjay, Kumar Naveen, Jindal Naresh, Bera Bidhan Chandra, Narang Gulshan, Mahajan Nand Kishore, Arora Devan, Anand Taruna, Vaid Rajesh Kumar, Yadav Mansi, Chandel Surender Singh, Malik Praveen, Tripathi Bhupendra Nath, Singh Raj Kumar

机构信息

ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.

出版信息

Comp Immunol Microbiol Infect Dis. 2016 Jun;46:60-5. doi: 10.1016/j.cimid.2016.04.001. Epub 2016 Apr 4.

DOI:10.1016/j.cimid.2016.04.001

PMID:27260812

Abstract

Swinepox virus (SWPV), a member of the genus Suipoxvirus causes generalized pock-like lesions on the body of domestic and wild pigs. Although outbreak has been reported in India since 1987, virus isolation and genetic characterization remained elusive. In September 2013, an outbreak of acute skin infection occurred in piglets in a commercial piggery unit at Rohtak district in Haryana, India. The presence of SWPV in scab samples collected from piglets succumbed to infection was confirmed by virus isolation, PCR amplification of SWPV-specific gene segments and nucleotide sequencing. Phylogenetic analysis of host-range genes of the SWPV revealed that the Indian isolate is genetically closely related to reference isolate SWPV/pig/U.S.A/1999/Nebraska. To the best of our knowledge this is the first report on isolation and genetic characterization of SWPV from pigs in India.

摘要

猪痘病毒（SWPV）是猪痘病毒属的成员，可在家猪和野猪身上引起全身性痘疹样病变。尽管自1987年以来印度已报告有疫情爆发，但病毒分离和基因特征分析一直难以实现。2013年9月，印度哈里亚纳邦罗塔克区的一个商业养猪场的仔猪发生了急性皮肤感染疫情。通过病毒分离、SWPV特异性基因片段的PCR扩增和核苷酸测序，证实了从死于感染的仔猪身上采集的痂皮样本中存在SWPV。对SWPV宿主范围基因的系统发育分析表明，印度分离株在基因上与参考分离株SWPV/猪/美国/1999/内布拉斯加州密切相关。据我们所知，这是印度首次关于从猪身上分离SWPV并进行基因特征分析的报告。