Richter Susanne, Schmoll Friedrich, Polzer Daniel, Leth Christoph, Revilla-Fernández Sandra, Schwarz Lukas, Auer Angelika, Sattler Tatjana
Institute for Veterinary Disease Control, AGES, Mödling, Austria.
Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, Vienna, Austria.
J Vet Diagn Invest. 2025 Aug 30:10406387251366960. doi: 10.1177/10406387251366960.
In 2008, nearly 50% of weaned piglets at a German 2-site production unit in Saxony-Anhalt had skin lesions 1-2 wk after relocation into the nursery. First clinical signs were maculae, followed by papules, pustules, and finally crusts, distributed over the dorsal and lateral body flank. Tentative clinical diagnosis was an infection with swinepox virus (SWPV; family , taxon species ). Electron microscopy confirmed within one hour that the causal agent was a brick-shaped poxvirus, and routine PCR validated the poxvirus detection; PCR for was negative. Phylogenetic analysis of the thymidine kinase genes from different poxviruses and from our SWPV isolates, 3 isolates from Germany, and 1 isolate from Austria, provided a good picture of evolutionary relationships of poxvirus genera, which was also consistent with phylogenetic analysis of poxviruses based on other genes. The German and Austrian isolates from domestic pigs were 99.8-100% identical to previously isolated German SWPV from wild boar and domestic pigs. All isolates belonged to the North American/European lineage. In a second step, SWPV assembly in naturally infected domestic pigs was analyzed by ultrathin sectioning. The virus assembly resembled that of other poxviruses and completed gaps in the SWPV morphogenesis model described in prior publications. Because there is no specific therapy, recommended interventions were improvements in biosecurity measures, especially hygiene management and disinfection procedures at the farm and within the transporters between the farrowing unit and the nursery. No further infections with SWPV were seen 5-6 wk after commencement of the hygiene interventions.
2008年，在萨克森 - 安哈尔特州一家德国双场地生产单位中，近50%的断奶仔猪在迁入保育舍1 - 2周后出现皮肤损伤。最初的临床症状为斑疹，随后是丘疹、脓疱，最后形成结痂，分布于背部和身体侧面。初步临床诊断为感染猪痘病毒（SWPV；痘病毒科，分类种 ）。电子显微镜在一小时内确认病原体是一种砖形痘病毒，常规PCR验证了痘病毒的检测结果；针对 的PCR检测为阴性。对来自不同痘病毒以及我们的SWPV分离株（3株来自德国，1株来自奥地利）的胸苷激酶基因进行系统发育分析，很好地展现了痘病毒属的进化关系，这也与基于其他基因的痘病毒系统发育分析结果一致。来自家猪的德国和奥地利分离株与先前从野猪和家猪中分离出的德国SWPV的相似度为99.8 - 100%。所有分离株均属于北美/欧洲谱系。第二步，通过超薄切片分析自然感染家猪中SWPV的装配情况。病毒装配过程与其他痘病毒相似，填补了先前出版物中描述的SWPV形态发生模型的空白。由于没有特效疗法，建议的干预措施是改善生物安全措施，特别是农场以及分娩舍和保育舍之间运输工具内的卫生管理和消毒程序。卫生干预措施开始5 - 6周后未再出现SWPV感染病例。
