Han Lu, Teng Yue-Wen, Feng Qing-Qing, Zhang Ren-Gang, Zhao Xing-Min, Lv Jie, Xie Bing
School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, People's Republic of China.
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China.
Curr Microbiol. 2016 Sep;73(3):386-392. doi: 10.1007/s00284-016-1074-0. Epub 2016 Jun 4.
An aerobic, gram-reaction-negative, rod-shaped bacterium, designated strain Z6(T), was isolated from sediment collected at Mohe Basin, China. And its taxonomic position was investigated by applying a polyphasic approach. Growth occurs at 10-45 °C (optimum, 30 °C), at pH 6.0-11.0 (optimum, pH 7.0) and in the presence of 0-3.0 % (w/v) NaCl (optimum, 0 %). The polar lipid profile of strain Z6(T) revealed the presence of diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and two unidentified phospholipids, and the major quinone was Q-10. The major fatty acids were C18:1 ω7c and/or C18:1 ω6c (summed feature 8) and C16:1 ω6c and/or C16:1 ω7c (summed feature 3). The predominant polyamine was homospermidine. The DNA G + C content of strain Z6(T) is 65.2 mol%. On the basis of the polyphasic evidence presented, strain Z6(T) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas mohensis sp. nov. is proposed. The type strain is Z6(T) (=CGMCC 1.12891(T) = JCM 19983(T)).
从中国漠河盆地采集的沉积物中分离出一株需氧、革兰氏阴性、杆状细菌,命名为菌株Z6(T)。采用多相分类法对其分类地位进行了研究。该菌株在10-45℃(最适温度30℃)、pH 6.0-11.0(最适pH 7.0)以及0-3.0%(w/v)NaCl(最适浓度0%)条件下生长。菌株Z6(T)的极性脂谱显示含有双磷脂酰甘油、磷脂酰乙醇胺、鞘糖脂和两种未鉴定的磷脂,主要醌类为Q-10。主要脂肪酸为C18:1 ω7c和/或C18:1 ω6c(总和特征8)以及C16:1 ω6c和/或C16:1 ω7c(总和特征3)。主要多胺为高亚精胺。菌株Z6(T)的DNA G + C含量为65.2 mol%。基于所提供的多相证据,菌株Z6(T)代表鞘氨醇单胞菌属的一个新物种,为此提出新名称漠河鞘氨醇单胞菌(Sphingomonas mohensis sp. nov.)。模式菌株为Z6(T)(=CGMCC 1.12891(T) = JCM 19983(T))。