Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
Institute of Bacteriology, Mycology and Hygiene, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1558-1563. doi: 10.1099/ijs.0.035964-0. Epub 2011 Aug 19.
An aerobic, Gram-negative-staining, motile, psychrophilic bacterium, designated strain S8-3(T), was isolated from alpine soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S8-3(T) was related to the genus Sphingomonas and had highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica S213(T) (98.0%). 16S RNA gene sequence similarity between strain S8-3(T) and Sphingomonas paucimobilis ATCC 29837(T) (the type species of the genus Sphingomonas) was 93.0%. Strain S8-3(T) contained Q-10 as the ubiquinone and C(18:1)ω7c (65.0%) and C(14:0) 2-OH (13.4%) as the dominant fatty acids (>10%). The major polyamines were the triamine sym-homospermidine and spermidine. The polar lipid profile contained sphingoglycolipid, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The DNA G+C content was 64.1 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain S8-3(T) is a representative of a novel species of the genus Sphingomonas, for which the name Sphingomonas alpina sp. nov. is proposed. The type strain is S8-3(T) (=DSM 22537(T)=LMG 26055(T)).
一株好氧、革兰氏阴性、运动、嗜冷的细菌，命名为 S8-3(T) 菌株，从高山土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，S8-3(T) 菌株与鞘氨醇单胞菌属密切相关，与 Sphingomonas oligophenolica S213(T)（模式种）的 16S rRNA 基因序列相似性最高，为 98.0%。S8-3(T) 菌株与 Sphingomonas paucimobilis ATCC 29837(T)（鞘氨醇单胞菌属的模式种）的 16S rRNA 基因序列相似性为 93.0%。S8-3(T) 菌株含有 Q-10 作为泛醌，C18:1ω7c（65.0%）和 C14:0 2-OH（13.4%）作为主要脂肪酸（>10%）。主要的多胺是三亚胺sym-homospermidine 和 spermidine。极性脂谱含有神经酰胺糖脂、磷脂酰甘油、双磷脂酰甘油、磷脂乙醇胺和磷脂单甲醚乙醇胺。DNA G+C 含量为 64.1mol%。表型、系统发育和 DNA-DNA 同源性研究的综合数据表明，S8-3(T) 菌株是鞘氨醇单胞菌属的一个新种的代表，提议将其命名为高山鞘氨醇单胞菌（ Sphingomonas alpina sp. nov.）。模式菌株是 S8-3(T)（=DSM 22537（T）=LMG 26055（T））。
