Dasgupta S, Mitra S
Proc Natl Acad Sci U S A. 1978 Jan;75(1):153-7. doi: 10.1073/pnas.75.1.153.
Nascent replicative form type II (RFII) DNA of coliphage M13 synthesized in an Escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith DNA polymerase I contains ribonucleotides that are retained in the covalently closed RFI DNA sealed in vitro by the joint action of T5 phage DNA polymerase and T4 phage DNA ligase. These RFI molecules are labile to alkali and RNase H, unlike the RFI produced either in vivo or from RFII with E. coli DNA polymerase I and E. coli DNA ligase. The ribonucleotides are located at one site and predominantly in one strand of the nascent RF DNA. Furthermore, these molecules contain multiple small gaps, randomly located, and one large gap in the intracistronic region.
在缺乏与DNA聚合酶I相关的5'至3'核酸外切酶的大肠杆菌突变体中合成的噬菌体M13的新生复制形式II（RFII）DNA含有核糖核苷酸，这些核糖核苷酸保留在通过T5噬菌体DNA聚合酶和T4噬菌体DNA连接酶的联合作用在体外密封的共价闭合RFI DNA中。与在体内产生的RFI或用大肠杆菌DNA聚合酶I和大肠杆菌DNA连接酶从RFII产生的RFI不同，这些RFI分子对碱和RNase H不稳定。核糖核苷酸位于一个位点，并且主要在新生RF DNA的一条链中。此外，这些分子包含多个随机定位的小间隙以及顺反子内区域中的一个大间隙。
