大肠杆菌K12的一种条件致死突变体，其在与DNA聚合酶I相关的5'至3'核酸外切酶方面存在缺陷。
A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I.
作者信息
Konrad E B, Lehman I R
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):2048-51. doi: 10.1073/pnas.71.5.2048.
Abstract
A mutant strain of E. coli, initially identified by an abnormally high frequency of recombination, has been found to be defective in the 5' --> 3' exonuclease associated with DNA polymerase I, but not in the polymerase activity. This defect is tolerated at 30 degrees , but is lethal at 43 degrees . Like other polymerase I mutants, the strain is unusually sensitive to methyl methanesulfonate and to ultraviolet irradiation; it is also unable to support the growth of phage lambda defective in general recombination, and shows a reduced rate of joining of 10S "Okazaki fragments." These results demonstrate that a functional DNA polymerase I is essential for normal growth and viability in E. coli K12.
摘要
一种最初通过异常高的重组频率鉴定出的大肠杆菌突变菌株，已被发现其与DNA聚合酶I相关的5'→3'核酸外切酶存在缺陷，但聚合酶活性无缺陷。这种缺陷在30摄氏度时可被耐受，但在43摄氏度时是致死的。与其他聚合酶I突变体一样，该菌株对甲基磺酸甲酯和紫外线照射异常敏感；它也无法支持在一般重组中存在缺陷的噬菌体λ的生长，并且10S“冈崎片段”的连接速率降低。这些结果表明，功能性的DNA聚合酶I对于大肠杆菌K12的正常生长和生存能力至关重要。
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本文引用的文献
1
A critical test of a current theory of genetic recombination in bacteriophage.对噬菌体中当前基因重组理论的一项关键测试。
Genetics. 1962 Feb;47(2):187-208. doi: 10.1093/genetics/47.2.187.
2
Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.大肠杆菌乳糖操纵子区域的转位。I. 乳糖操纵子的倒位及噬菌体φ80介导的乳糖转导
J Mol Biol. 1966 Aug;19(2):254-65. doi: 10.1016/s0022-2836(66)80003-7.
3
An active fragment of DNA polymerase produced by proteolytic cleavage.通过蛋白水解切割产生的DNA聚合酶活性片段。
Biochem Biophys Res Commun. 1969 Dec 4;37(6):982-9. doi: 10.1016/0006-291x(69)90228-9.
4
Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.DNA聚合酶I缺陷型大肠杆菌突变体中新复制DNA链的缓慢连接。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2954-7. doi: 10.1073/pnas.68.12.2954.
5
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Nat New Biol. 1971 Oct 27;233(43):281-3. doi: 10.1038/newbio233281a0.
6
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J Bacteriol. 1970 Nov;104(2):871-81. doi: 10.1128/jb.104.2.871-881.1970.
7
A possible function of DNA polymerase in chromosome replication.DNA聚合酶在染色体复制中的一种可能功能。
Biochem Biophys Res Commun. 1970 Nov 25;41(4):973-80. doi: 10.1016/0006-291x(70)90180-4.
8
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9
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