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1
A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I.大肠杆菌K12的一种条件致死突变体，其在与DNA聚合酶I相关的5'至3'核酸外切酶方面存在缺陷。
Proc Natl Acad Sci U S A. 1974 May;71(5):2048-51. doi: 10.1073/pnas.71.5.2048.
2
Some properties of mutants of Escherichia coli K12 defective in DNA polymerase I and exonuclease V.大肠杆菌K12中DNA聚合酶I和核酸外切酶V缺陷型突变体的一些特性
Mol Gen Genet. 1974 Apr 9;130(1):29-38. doi: 10.1007/BF00270516.
3
Biochemical characterization of mutant forms of DNA polymerase I from Escherichia coli. II. The polAex1 mutation.大肠杆菌DNA聚合酶I突变形式的生化特性。II. polAex1突变
J Biol Chem. 1976 Jul 10;251(13):4085-9.
4
Degradation of DNA following ultraviolet irradiation of Escherichia coli K12 mutants lacking DNA polymerase I and exonuclease V.缺乏DNA聚合酶I和核酸外切酶V的大肠杆菌K12突变体经紫外线照射后DNA的降解
Mol Gen Genet. 1974 Apr 9;130(1):39-45. doi: 10.1007/BF00270517.
5
Excision of pyrimidine dimers in normal and T4-infected Escherichia coli: effect of polA and other mutations.正常及T4噬菌体感染的大肠杆菌中嘧啶二聚体的切除：polA及其他突变的影响
Biochim Biophys Acta. 1975 Mar 10;383(2):188-94. doi: 10.1016/0005-2787(75)90260-9.
6
A mutant of Escherichia coli defective in DNA polymerase II activity.一种DNA聚合酶II活性存在缺陷的大肠杆菌突变体。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3238-42. doi: 10.1073/pnas.69.11.3238.
7
Involvement of Escherichia coli DNA polymerase-I-associated 5' in equilibrium 3' exonuclease in excision-repair of UV-damaged DNA.大肠杆菌DNA聚合酶I相关的5'平衡3'核酸外切酶参与紫外线损伤DNA的切除修复
Basic Life Sci. 1975;5A:219-23. doi: 10.1007/978-1-4684-2895-7_29.
8
Biochemical characterization of mutant forms of DNA polymerase I from Escherichia coli. I. The polA12 mutation.大肠杆菌DNA聚合酶I突变形式的生化特性。I. polA12突变
J Biol Chem. 1976 Jul 10;251(13):4078-84.
9
Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.转录终止因子rho缺陷的大肠杆菌条件致死突变体的分离与鉴定。
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1959-63. doi: 10.1073/pnas.73.6.1959.
10
Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I.在缺乏脱氧核糖核酸聚合酶I的大肠杆菌菌株中，包含uvrB的缺失的条件致死性。
J Bacteriol. 1972 Nov;112(2):676-81. doi: 10.1128/jb.112.2.676-681.1972.

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1
Fast evolution of SOS-independent multi-drug resistance in bacteria.细菌中不依赖SOS的多药耐药性的快速进化。
Elife. 2025 Jul 9;13:RP95058. doi: 10.7554/eLife.95058.
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The DNA Exonucleases of Escherichia coli.大肠杆菌的DNA外切核酸酶
EcoSal Plus. 2011 Dec;4(2). doi: 10.1128/ecosalplus.4.4.7.
3
DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present.DNA 聚合酶推动了 DNA 合成测序技术：过去和现在都是如此。
Front Microbiol. 2014 Jun 24;5:305. doi: 10.3389/fmicb.2014.00305. eCollection 2014.
4
Hyper-recombination and mutator effects of the mms9-1, mms13-1, and mms21-1 mutations in Saccharomyces cerevisiae.在酿酒酵母中，mms9-1、mms13-1 和 mms21-1 突变的超重组和诱变效应。
Curr Genet. 1981 Dec;4(3):223-32. doi: 10.1007/BF00420503.
5
Plasmid cloning and expression of the E. coli polA (+) gene in S. cerevisiae.在酿酒酵母中克隆和表达大肠杆菌 polA（+）基因的质粒。
Curr Genet. 1984 Jul;8(5):333-40. doi: 10.1007/BF00419821.
6
Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming.遗传学和生物化学分析揭示了复制重启蛋白在II类内含子逆转归巢中的关键作用。
PLoS Genet. 2013 Apr;9(4):e1003469. doi: 10.1371/journal.pgen.1003469. Epub 2013 Apr 25.
7
The rate of polymerase release upon filling the gap between Okazaki fragments is inadequate to support cycling during lagging strand synthesis.在填补冈崎片段之间的间隙时，聚合酶的释放速度不足以支持滞后链合成过程中的循环。
J Mol Biol. 2011 Nov 18;414(1):15-27. doi: 10.1016/j.jmb.2011.09.039. Epub 2011 Oct 1.
8
Oxidative stress resistance in Deinococcus radiodurans.耐辐射球菌的抗氧化应激能力。
Microbiol Mol Biol Rev. 2011 Mar;75(1):133-91. doi: 10.1128/MMBR.00015-10.
9
Homologous recombination prevents methylation-induced toxicity in Escherichia coli.同源重组可预防大肠杆菌中甲基化诱导的毒性。
Nucleic Acids Res. 2006 May 2;34(8):2258-68. doi: 10.1093/nar/gkl222. Print 2006.
10
The nature of the 5'-terminus is a major determinant for DNA processing by Schizosaccharomyces pombe Rad2p, a FEN-1 family nuclease.5'-末端的性质是粟酒裂殖酵母Rad2p（一种FEN-1家族核酸酶）进行DNA加工的主要决定因素。
Nucleic Acids Res. 2000 Aug 1;28(15):2893-901. doi: 10.1093/nar/28.15.2893.

本文引用的文献

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A critical test of a current theory of genetic recombination in bacteriophage.对噬菌体中当前基因重组理论的一项关键测试。
Genetics. 1962 Feb;47(2):187-208. doi: 10.1093/genetics/47.2.187.
2
Transposition of the lac region of Escherichia coli. I. Inversion of the lac operon and transduction of lac by phi80.大肠杆菌乳糖操纵子区域的转位。I. 乳糖操纵子的倒位及噬菌体φ80介导的乳糖转导
J Mol Biol. 1966 Aug;19(2):254-65. doi: 10.1016/s0022-2836(66)80003-7.
3
An active fragment of DNA polymerase produced by proteolytic cleavage.通过蛋白水解切割产生的DNA聚合酶活性片段。
Biochem Biophys Res Commun. 1969 Dec 4;37(6):982-9. doi: 10.1016/0006-291x(69)90228-9.
4
Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.DNA聚合酶I缺陷型大肠杆菌突变体中新复制DNA链的缓慢连接。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2954-7. doi: 10.1073/pnas.68.12.2954.
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DNA synthesis in lysates of RecB- and Rec+ E. coli cells.RecB 型和 Rec+ 型大肠杆菌细胞裂解物中的 DNA 合成。
Nat New Biol. 1971 Oct 27;233(43):281-3. doi: 10.1038/newbio233281a0.
6
Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis.大肠杆菌修复合成缺陷的X射线敏感突变体的遗传和分子特征
J Bacteriol. 1970 Nov;104(2):871-81. doi: 10.1128/jb.104.2.871-881.1970.
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A possible function of DNA polymerase in chromosome replication.DNA聚合酶在染色体复制中的一种可能功能。
Biochem Biophys Res Commun. 1970 Nov 25;41(4):973-80. doi: 10.1016/0006-291x(70)90180-4.
8
Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis.通过有限蛋白酶解选择性去除大肠杆菌B中脱氧核糖核酸聚合酶的核酸外切酶活性。
Proc Natl Acad Sci U S A. 1970 Jan;65(1):168-75. doi: 10.1073/pnas.65.1.168.
9
Isolation of an E. coli strain with a mutation affecting DNA polymerase.分离出一株具有影响DNA聚合酶突变的大肠杆菌菌株。
Nature. 1969 Dec 20;224(5225):1164-6. doi: 10.1038/2241164a0.
10
Persistence of deoxyribonucleic acid polymerase I and its 5'--3' exonuclease activity in PolA mutants of Escherichia coli K12.大肠杆菌K12的PolA突变体中脱氧核糖核酸聚合酶I及其5'→3'核酸外切酶活性的持续性
J Biol Chem. 1973 Nov 25;248(22):7717-23.

大肠杆菌K12的一种条件致死突变体，其在与DNA聚合酶I相关的5'至3'核酸外切酶方面存在缺陷。

A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I.

作者信息

Konrad E B, Lehman I R

出版信息

Proc Natl Acad Sci U S A. 1974 May;71(5):2048-51. doi: 10.1073/pnas.71.5.2048.

DOI:10.1073/pnas.71.5.2048
PMID:4600786
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388383/
Abstract

A mutant strain of E. coli, initially identified by an abnormally high frequency of recombination, has been found to be defective in the 5' --> 3' exonuclease associated with DNA polymerase I, but not in the polymerase activity. This defect is tolerated at 30 degrees , but is lethal at 43 degrees . Like other polymerase I mutants, the strain is unusually sensitive to methyl methanesulfonate and to ultraviolet irradiation; it is also unable to support the growth of phage lambda defective in general recombination, and shows a reduced rate of joining of 10S "Okazaki fragments." These results demonstrate that a functional DNA polymerase I is essential for normal growth and viability in E. coli K12.

摘要

一种最初通过异常高的重组频率鉴定出的大肠杆菌突变菌株，已被发现其与DNA聚合酶I相关的5'→3'核酸外切酶存在缺陷，但聚合酶活性无缺陷。这种缺陷在30摄氏度时可被耐受，但在43摄氏度时是致死的。与其他聚合酶I突变体一样，该菌株对甲基磺酸甲酯和紫外线照射异常敏感；它也无法支持在一般重组中存在缺陷的噬菌体λ的生长，并且10S“冈崎片段”的连接速率降低。这些结果表明，功能性的DNA聚合酶I对于大肠杆菌K12的正常生长和生存能力至关重要。