大肠杆菌素E1质粒DNA在细胞提取物中的复制。II. 早期复制中间体的选择性合成。
Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates.
作者信息
Sakakibara Y, Tomizawa J
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1403-7. doi: 10.1073/pnas.71.4.1403.
Abstract
The major products of colicin E1 plasmid DNA synthesis in cell extracts are completely replicated molecules and a class of molecules containing newly synthesized small DNA fragments. The addition of 10% glycerol and/or 2 mM spermidine to extracts blocks synthesis of completely replicated molecules while enhancing synthesis of molecules containing newly synthesized DNA fragments. The latter molecules, which contain on the average two DNA fragments of approximately 6 S, are early replicative intermediates for synthesis of completely replicated molecules. Synthesis of the intermediates is sensitive to rifampicin and depends on RNA synthesis. RNA components are linked to the 6S DNA molecules.
摘要
细胞提取物中大肠杆菌素E1质粒DNA合成的主要产物是完全复制的分子和一类含有新合成小DNA片段的分子。向提取物中添加10%甘油和/或2 mM亚精胺会阻断完全复制分子的合成，同时增强含有新合成DNA片段的分子的合成。后一类分子平均含有两个约6S的DNA片段，是完全复制分子合成的早期复制中间体。中间体的合成对利福平敏感，且依赖于RNA合成。RNA成分与6S DNA分子相连。
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