Dulgheroff Ana Carolina Bernardes, Silva George Allan Villarouco da, Naveca Felipe Gomes, Oliveira Adriana Gonçalves de, Domingues André Luiz da Silva
Laboratory of Microbiology, Institute of Biological and Natural Science, Federal University of Triângulo Mineiro, Uberaba, Minas Gerais, Brazil; Health Technical School, Health Science Center, Federal University of Paraíba, João Pessoa, Paraíba, Brazil.
Laboratory of Infectious Disease Ecology in the Amazon, Leonidas e Maria Deane Institute, Oswaldo Cruz Foundation, Manaus, Amazonas, Brazil.
Braz J Microbiol. 2016 Jul-Sep;47(3):731-40. doi: 10.1016/j.bjm.2016.04.012. Epub 2016 May 7.
Group A rotaviruses are the main causative agent of infantile gastroenteritis. The segmented nature of the viral genome allows reassortment of genome segments, which can generate genetic variants. In this study, we characterized the diversity of the VP7, VP4 (VP8*), VP6, NSP4, and NSP5 genes of the rotaviruses that circulated from 2005 to 2011 in the Triângulo Mineiro (TM) region of Brazil. Samples with genotypes G2 (sublineages IVa-1 and IVa-3), G1 (sublineage I-A), G9 (lineage III), G12 (lineages II and III), G8 (lineage II), G3 (lineage III), P[4] (sublineages IVa and IVb), P[8] (sublineages P[8]-3.6, P[8]-3.3, and P[8]-3.1), I2 (lineage VII), E2 (lineages VI, XII, and X), and H2 (lineage III) were identified. The associations found in the samples were G1, G9, or G12 with P[8]-I1-E1-H1; G2 or G8 with P[4]-I2-E2-H2; G12 with I3-E3-H6; and G3 with P[4]-I2-E3-H3 (previously unreported combination). Reassortment events in G2P[4] strains and an apparent pattern of temporal segregation within the lineages were observed. Five TM samples contained genes that exhibited high nucleotide and amino acid identities with strains of animal origin. The present study includes a period of pre- and post-introduction of rotavirus vaccination in all Brazilian territories, thereby serving as a basis for monitoring changes in the genetic constitution of rotaviruses. The results also contribute to the understanding of the diversity and evolution of rotaviruses in a global context.
A组轮状病毒是婴幼儿肠胃炎的主要病原体。病毒基因组的分段性质允许基因组片段进行重配，从而产生遗传变异体。在本研究中，我们对2005年至2011年在巴西米纳斯吉拉斯州三角地区（TM）流行的轮状病毒的VP7、VP4（VP8*）、VP6、NSP4和NSP5基因的多样性进行了表征。鉴定出了具有G2（IVa-1和IVa-3亚系）、G1（I-A亚系）、G9（III系）、G12（II和III系）、G8（II系）、G3（III系）、P[4]（IVa和IVb亚系）、P[8]（P[8]-3.6、P[8]-3.3和P[8]-3.1亚系）、I2（VII系）、E2（VI、XII和X系）和H2（III系）基因型的样本。在样本中发现的组合为：G1、G9或G12与P[8]-I1-E1-H1；G2或G8与P[4]-I2-E2-H2；G12与I3-E3-H6；以及G3与P[4]-I2-E3-H3（以前未报道的组合）。观察到G2P[4]毒株中的重配事件以及各谱系内明显的时间分离模式。五个TM样本所含基因与动物源毒株具有高度的核苷酸和氨基酸同一性。本研究涵盖了巴西所有地区轮状病毒疫苗引入前后的时期，从而为监测轮状病毒基因构成的变化提供了依据。研究结果也有助于在全球背景下理解轮状病毒 的多样性和进化。
