National Engineering Research Center for Nanomedicine, Key Laboratory of Molecular Biophysics of the Ministry of Education, Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan, Hubei 430074, People's Republic of China.
Infinitus (China) Company Ltd. , Guangzhou, Guangdong 510665, People's Republic of China.
J Agric Food Chem. 2016 Jun 29;64(25):5207-14. doi: 10.1021/acs.jafc.6b02183. Epub 2016 Jun 15.
Hesperidin, abundant in citrus fruits, has a wide range of pharmacological effects, including anticarcinogenic, anti-inflammatory, antioxidative, radioprotective, and antiviral activities. However, relatively few studies on the effects of hesperidin on lipid metabolism have been reported. Here, using Caenorhaditis elegans as a model animal, we found that 100 μM hesperidin significantly decreased fat accumulation in both high-fat worms cultured in nematode growth medium containing 10 mM glucose (83.5 ± 1.2% versus control by Sudan Black B staining and 87.6 ± 2.0% versus control by Oil Red O staining; p < 0.001) and daf-2 mutant worms (87.8 ± 1.4% versus control by Oil Red O staining; p < 0.001). Furthermore, 50 μM hesperidin decreased the ratio of oleic acid/stearic acid (C18:1Δ9/C18:0) (p < 0.05), and supplementation of oleic acid could restore the inhibitory effect of hesperidin on fat accumulation. Hesperidin significantly downregulated the expression of stearoyl-CoA desaturase, fat-6, and fat-7 (p < 0.05), and mutation of fat-6 and fat-7 reversed fat accumulation inhibited by hesperidin. In addition, hesperidin decreased the expression of other genes involved in lipid metabolism, including pod-2, mdt-15, acs-2, and kat-1 (p < 0.05). These results suggested that hesperidin reduced fat accumulation by affecting several lipid metabolism pathways, such as fat-6 and fat-7. This study provided new insights into elucidating the mechanism underlying the regulation of lipid metabolism by hesperidin.
橙皮苷在柑橘类水果中含量丰富，具有广泛的药理作用，包括抗癌、抗炎、抗氧化、放射保护和抗病毒活性。然而，关于橙皮苷对脂质代谢影响的研究相对较少。在这里，我们使用秀丽隐杆线虫作为模型动物，发现 100μM 的橙皮苷可显著减少高糖（含 10mM 葡萄糖的线虫生长培养基）培养的高脂肪线虫（苏丹黑 B 染色 83.5±1.2%，油红 O 染色 87.6±2.0%，均显著低于对照组，p<0.001）和 daf-2 突变线虫（油红 O 染色 87.8±1.4%，显著低于对照组，p<0.001）中的脂肪积累。此外，50μM 的橙皮苷降低了油酸/硬脂酸（C18:1Δ9/C18:0）的比值（p<0.05），补充油酸可以恢复橙皮苷对脂肪积累的抑制作用。橙皮苷显著下调硬脂酰辅酶 A 去饱和酶、fat-6 和 fat-7 的表达（p<0.05），fat-6 和 fat-7 的突变逆转了橙皮苷抑制脂肪积累的作用。此外，橙皮苷还降低了参与脂质代谢的其他基因的表达，包括 pod-2、mdt-15、acs-2 和 kat-1（p<0.05）。这些结果表明，橙皮苷通过影响 fat-6 和 fat-7 等几条脂质代谢途径减少脂肪积累。本研究为阐明橙皮苷调节脂质代谢的机制提供了新的见解。
