Fakhry Amal Bahgat, Ahmed Asmaa Ismail, AbdelAlim Mahmoud Abdo, Ramadan Dalia Ibrahim
Department of Clinical and Chemical Pathology, Kasr Al-Aini School of Medicine, Cairo University, Cairo, Egypt E-mail :
Asian Pac J Cancer Prev. 2016;17(5):2383-8.
The reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) gene is a novel transformation suppressor gene that was linked to several malignancies.
To analyze any association between RECK gene rs10814325 single-nucleotide polymorphism (SNP) and HCC susceptibility along with it is association wiht various clinico-pathological and laboratory data.
RECK gene rs10814325 SNP was estimated, using real-time PCR technique, in 30 HCC patients on top of chronic HCV infection, 30 HCV related cirrhotic patients and 30 healthy controls.
No special pattern of association could be detected on comparing the RECK gene rs10814325 genotypes(P=0.5), or alleles(P=0.49) among the studied groups. HCC patients with TT genotype had younger age (mean of 54.1±6.0 years vs 60.6±10.2 years for TC/CC genotypes, P=0.035). Abdominal distension was significantly greater in TT genotype patients (75% vs 30% of TC/CC genotypes, P=0.045). TT genotype was present in 75% of patients with lymph node metastasis. Serum GGT levels were higher in TT genotype patients [80 of (48.5-134.8) vs 40 IU/l (33-87.5) for TC/CCgenotypes], and lower limb edema was observed in 60% of TT vs 20% of TC/CCgenotypes, however, both just failed to reach significance (P=0.05 and P=0.06, respectively).
RECK gene rs10814325 T>C could not be considered a risk factor for HCC development on top of HCV, but may be related to the disease progression and metastasis.
含Kazal基序的逆转录诱导富含半胱氨酸蛋白（RECK）基因是一种新型的肿瘤抑制基因，与多种恶性肿瘤相关。
分析RECK基因rs10814325单核苷酸多态性（SNP）与肝癌易感性之间的关联，以及其与各种临床病理和实验室数据的相关性。
采用实时荧光定量PCR技术，对30例慢性丙型肝炎病毒（HCV）感染的肝癌患者、30例HCV相关肝硬化患者和30例健康对照者进行RECK基因rs10814325 SNP检测。
在研究组中，比较RECK基因rs10814325基因型（P=0.5）或等位基因（P=0.49）时，未发现特殊的关联模式。TT基因型的肝癌患者年龄较轻（平均54.1±6.0岁，而TC/CC基因型为60.6±10.2岁，P=0.035）。TT基因型患者腹胀明显更常见（75%，而TC/CC基因型为30%，P=0.045）。75%有淋巴结转移的患者为TT基因型。TT基因型患者血清γ-谷氨酰转移酶（GGT）水平较高[80（48.5-134.8），而TC/CC基因型为40 IU/L（33-87.5）]，TT基因型患者下肢水肿发生率为60%，而TC/CC基因型为20%，但两者均未达到统计学显著性（分别为P=0.05和P=0.06）。
RECK基因rs10814325 T>C不能被视为HCV感染基础上肝癌发生的危险因素，但可能与疾病进展和转移有关。
