Hepatology, Gastroenterology, and Infectious Diseases Department, Faculty of Medicine, Benha University, Benha, Egypt.
Benha Fever Hospital, Benha, Egypt.
Arab J Gastroenterol. 2022 Aug;23(3):201-205. doi: 10.1016/j.ajg.2022.05.001. Epub 2022 Aug 5.
Chronic hepatitis B (CHB) infection is a major risk factor for hepatocellular carcinoma (HCC). The RECK gene is a critical tumor suppressor gene. This study aimed to assess the association between RECK gene single nucleotide polymorphisms (SNPs) and the development of HCC in Egyptian patients with chronic hepatitis B.
In this case-control study, we enrolled patients with CHB from the Gastroenterology Department, Benha University, from June 2016 to February 2018. The RECK gene SNP rs10814325 was identified using real-time PCR allelic discrimination via TaqMan SNP genotyping assays (Applied Biosystems, USA).
We enrolled 140 participants in this study. The participants were divided into Group I, which comprised 50 participants with CHB only, Group II, which comprised 50 participants with CHB and HCC, and Group III, which comprised 40 healthy participants. A significantly higher hepatitis B virus DNA viremia level was found in patients with HCC. The predominant RECK genotype was the T/T allele, followed by the T/C allele; however, no significant difference in the distribution of RECK gene SNPs was found between the study groups. No statistically significant difference in RECK gene SNPs was reported among patients with HCC of different Child classes or based on the number, site, size of HCC, and lymph node involvement. Receiver operating characteristic curves showed that a serum alpha-fetoprotein level of 92 ng/ml was 96 % sensitive and 100 % specific for the detection of HCC, with an area under the operating characteristic curve of 0.98.
RECK gene SNPs have no significant association with the development and characteristics of hepatitis B-related HCC in Egyptian patients.
慢性乙型肝炎（CHB）感染是肝细胞癌（HCC）的一个主要危险因素。RECK 基因是一个重要的肿瘤抑制基因。本研究旨在评估 RECK 基因单核苷酸多态性（SNP）与埃及慢性乙型肝炎患者 HCC 发生的相关性。
在这项病例对照研究中，我们招募了 2016 年 6 月至 2018 年 2 月在 Benha 大学胃肠病科就诊的 CHB 患者。使用 TaqMan SNP 基因分型检测（Applied Biosystems，美国）通过实时 PCR 等位基因鉴别来鉴定 RECK 基因 SNP rs10814325。
我们共纳入了 140 名参与者。参与者被分为三组：第 I 组由 50 名仅患有 CHB 的患者组成，第 II 组由 50 名患有 CHB 合并 HCC 的患者组成，第 III 组由 40 名健康参与者组成。患有 HCC 的患者乙型肝炎病毒 DNA 血症水平显著升高。RECK 基因的主要基因型是 T/T 等位基因，其次是 T/C 等位基因；然而，在研究组之间，RECK 基因 SNP 的分布没有显著差异。不同 Child 分级的 HCC 患者或根据 HCC 的数量、部位、大小以及淋巴结受累情况，RECK 基因 SNP 无统计学差异。受试者工作特征曲线显示，血清甲胎蛋白水平为 92ng/ml 时，对 HCC 的检测具有 96%的敏感性和 100%的特异性，曲线下面积为 0.98。
RECK 基因 SNP 与埃及慢性乙型肝炎患者乙型肝炎相关 HCC 的发生和特征无显著相关性。
