中国哺乳期母亲母乳中多不饱和脂肪酸与脂肪酸去饱和酶基因多态性的关联

Association of polyunsaturated fatty acids in breast milk with fatty acid desaturase gene polymorphisms among Chinese lactating mothers.

作者信息

Ding Zhen, Liu Guo-Liang, Li Xiang, Chen Xue-Yan, Wu Yi-Xia, Cui Can-Can, Zhang Xi, Yang Guang, Xie Lin

机构信息

Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Chang Chun 130021, China.

Experimental Center of Prevention, School of Public Health, Jilin University, Chang Chun 130021, China.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 2016 Jun;109:66-71. doi: 10.1016/j.plefa.2016.03.009. Epub 2016 Mar 23.

DOI:10.1016/j.plefa.2016.03.009

PMID:27269715

Abstract

BACKGROUND

The fatty acid desaturase (FADS) controls polyunsaturated fatty acid (PUFA) synthesis in human tissues and breast milk.

DESIGN

Evaluate the influence of 10 single nucleotide polymorphisms (SNPs) and various haplotypes in the FADS gene cluster (FADS1, FADS2, FADS3) on PUFA concentration in the breast milk of 209 healthy Chinese women. PUFA concentrations were measured in breast milk using gas chromatography and genotyping was performed using the Sequenom Mass Array system.

RESULTS

A SNP (rs1535) and 2-locus haplotypes (rs3834458-rs1535, rs1535-rs174575) in the FADS2 gene were associated with concentrations of γ-linoleic acid (GLA) and arachidonic acid (AA) in breast milk. Likewise, in the FADS1 gene, a 2-locus constructed haplotype (rs174547-rs174553) also affected GLA and AA concentration (P<0.05 for all). Minor allele carriers of the SNP and haplotypes described above had lower concentrations of GLA and AA. In the FADS2 gene, the 3-locus haplotype rs3834458-rs1535-rs174575, significantly affected concentrations of GLA but not AA. Pairwise comparison showed that individuals major homozygous for the SNP rs1000778 in the FADS3 gene had lower concentrations of ALA and linoleic acid (LA) in their breast milk.

CONCLUSION

Polymorphisms in the FADS gene cluster influence PUFA concentrations in the breast milk of Chinese Han lactating women.

摘要

背景

脂肪酸去饱和酶（FADS）控制人体组织和母乳中多不饱和脂肪酸（PUFA）的合成。

设计

评估FADS基因簇（FADS1、FADS2、FADS3）中的10个单核苷酸多态性（SNP）和各种单倍型对209名健康中国女性母乳中PUFA浓度的影响。使用气相色谱法测量母乳中的PUFA浓度，并使用Sequenom Mass Array系统进行基因分型。

结果

FADS2基因中的一个SNP（rs1535）和两个位点的单倍型（rs3834458 - rs1535、rs1535 - rs174575）与母乳中γ-亚麻酸（GLA）和花生四烯酸（AA）的浓度相关。同样，在FADS1基因中，一个构建的两个位点的单倍型（rs174547 - rs174553）也影响GLA和AA的浓度（所有P<0.05）。上述SNP和单倍型的次要等位基因携带者的GLA和AA浓度较低。在FADS2基因中，三个位点的单倍型rs3834458 - rs1535 - rs174575显著影响GLA的浓度，但不影响AA的浓度。成对比较显示，FADS3基因中SNP rs1000778的主要纯合个体母乳中α-亚麻酸（ALA）和亚油酸（LA）的浓度较低。