利用荧光显微镜对细胞中的膜蛋白相互作用进行研究。
Investigations of membrane protein interactions in cells using fluorescence microscopy.
作者信息
Abouelkheir Mahmoud, Roy Tanaya, Krzyscik Mateusz A, Özdemir Ece, Hristova Kalina
机构信息
Department of Materials Science and Engineering and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore MD 21218, USA; Chemistry-Biology Interface Program, Johns Hopkins University, Baltimore MD 21218, USA.
Department of Materials Science and Engineering and Institute for NanoBioTechnology, Johns Hopkins University, Baltimore MD 21218, USA.
出版信息
Curr Opin Struct Biol. 2024 Jun;86:102816. doi: 10.1016/j.sbi.2024.102816. Epub 2024 Apr 21.
The interactions between proteins in membranes govern many cellular functions. Our ability to probe for such interactions has greatly evolved in recent years due to the introduction of new fluorescence techniques. As a result, we currently have a choice of methods that can be used to assess the spatial distribution of a membrane protein, its association state, and the thermodynamic stability of the oligomers in the native milieu. These biophysical measurements have revealed new insights into important biological processes in cellular membranes.
膜中蛋白质之间的相互作用支配着许多细胞功能。近年来，由于新荧光技术的引入，我们探测此类相互作用的能力有了很大发展。因此，我们目前有多种方法可供选择，可用于评估膜蛋白的空间分布、其缔合状态以及天然环境中寡聚体的热力学稳定性。这些生物物理测量揭示了关于细胞膜中重要生物学过程的新见解。
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