用于膜蛋白结构与功能研究的纳米圆盘
Nanodiscs for structural and functional studies of membrane proteins.
作者信息
Denisov Ilia G, Sligar Stephen G
机构信息
Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
出版信息
Nat Struct Mol Biol. 2016 Jun;23(6):481-6. doi: 10.1038/nsmb.3195. Epub 2016 Jun 7.
Abstract
Membrane proteins have long presented a challenge to biochemical and functional studies. In the absence of a bilayer environment, individual proteins and critical macromolecular complexes may be insoluble and may display altered or absent activities. Nanodisc technology provides important advantages for the isolation, purification, structural resolution and functional characterization of membrane proteins. In addition, the ability to precisely control the nanodisc composition provides a nanoscale membrane surface for investigating molecular recognition events.
摘要
长期以来，膜蛋白一直是生物化学和功能研究面临的挑战。在没有双层膜环境的情况下，单个蛋白质和关键的大分子复合物可能不溶，并可能表现出改变的活性或无活性。纳米圆盘技术为膜蛋白的分离、纯化、结构解析和功能表征提供了重要优势。此外，精确控制纳米圆盘组成的能力为研究分子识别事件提供了一个纳米级的膜表面。
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