Shukla Pushpendra Kumar, Misra Ankita, Srivastava Sharad, Rawat Ajay K S
Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
Pharmacogn Mag. 2016 May;12(Suppl 2):S159-64. doi: 10.4103/0973-1296.182173. Epub 2016 May 11.
Different Leucas species are well known as "Dronpushpi," a well-known herb of Ayurveda, used in the treatment of various ailments.
Evaluation of four industrially important Leucas species for their in vitro antidiabetic potential and radical scavenging effect along with high-performance liquid chromatographic quantification of the bioactive triterpenes.
The quantification of triterpenes was carried out on C-18 column with acetonitrile and water (90:10) as the solvent system at a detection wavelength of 210 nm. In vitro antidiabetic activity was evaluated by α-amylase inhibition assay based on starch-iodine and 3,5 dinitrosalicylic acid (DNS) method. Antioxidant activity was calculated by five different models, namely total phenolic and total flavonoid content, free radical scavenging activity by 1-1-diphenyl-2-pic-rylhydrazyl (DPPH), ferric-reducing power assay, and the total antioxidant capacity.
Maximum concentration of oleanolic acid was found in Leucas cristata, followed by Leucas mollissima, Leucas Aspera, and Leucas biflora. Ursolic acid was highest in L. mollissima and then in L. biflora, L. cristata, and L. aspera, respectively. In in vitro antidiabetic activity, IC50 of L. aspera (1.56 ± 0.01 mg/ml) and L. mollissima (0.75 ± 0.005 mg/ml) were found to be highest in DNS and iodine starch assay. IC50 in DPPH assay ranges from 0.6 ± 0.011 to 1.68 ± 0.011 mg/ml. Antioxidant capacity follows the order; L. aspera > L. mollissima > L. biflora > L. cristata.
Promising activities were observed in targeted species, thus L. mollissima, L. biflora, and L. cristata can be used alternatively as a substitute to L. aspera.
Physicochemical parameters are within the limit as per the Ayurvedic Pharmacopoeia of IndiaMaximum concentration of oleanolic acid was found in Leucas cristata; however, ursolic acid was highest in Leucas mollissima In vitro antidiabetic activity of Leucas aspera and L. mollissima was found to be heighest as compared to other species. However, antioxidant capacity is almost similar in targeted species.Promising activities were observed in all the species, thus L. mollissima, Leucas biflora, and L. cristata can be used alternatively as a substitute to L. aspera.
不同的香科科属植物以“Dronpushpi”而闻名，这是阿育吠陀医学中一种著名的草药，用于治疗各种疾病。
评估四种具有重要工业价值的香科科属植物的体外抗糖尿病潜力和自由基清除作用，并对生物活性三萜进行高效液相色谱定量分析。
在C-18柱上，以乙腈和水（90:10）为溶剂系统，在210nm检测波长下进行三萜的定量分析。通过基于淀粉-碘和3,5-二硝基水杨酸（DNS）法的α-淀粉酶抑制试验评估体外抗糖尿病活性。通过五种不同模型计算抗氧化活性，即总酚和总黄酮含量、1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、铁还原能力测定和总抗氧化能力。
在毛叶香科科中发现齐墩果酸的最大浓度，其次是柔毛香科科、粗糙香科科和二花香科科。熊果酸在柔毛香科科中含量最高，然后分别在二花香科科、毛叶香科科和粗糙香科科中含量较高。在体外抗糖尿病活性方面，在DNS和碘淀粉试验中，粗糙香科科（1.56±0.01mg/ml）和柔毛香科科（0.75±0.005mg/ml）的IC50最高。DPPH试验中的IC50范围为0.6±0.011至1.68±0.011mg/ml。抗氧化能力顺序为：粗糙香科科＞柔毛香科科＞二花香科科＞毛叶香科科。
在目标物种中观察到有前景的活性，因此，柔毛香科科、二花香科科和毛叶香科科可替代粗糙香科科使用。
理化参数在印度阿育吠陀药典规定的范围内。在毛叶香科科中发现齐墩果酸的最大浓度；然而，熊果酸在柔毛香科科中含量最高。与其他物种相比，粗糙香科科和柔毛香科科的体外抗糖尿病活性最高。然而，目标物种的抗氧化能力几乎相似。在所有物种中都观察到有前景的活性，因此，柔毛香科科、二花香科科和毛叶香科科可替代粗糙香科科使用。
