Abe Shigeaki, Seitoku Eri, Iwadera Nobuki, Hamba Yusuke, Yamagata Shuichi, Akasaka Tsukasa, Kusaka Teruo, Inoue Satoshi, Yawaka Yasutaka, Iida Junichiro, Sano Hidehiko, Yonezawa Tetsu, Yoshida Yasuhiro
J Biomed Nanotechnol. 2016 Mar;12(3):472-80. doi: 10.1166/jbn.2016.2190.
We assessed the biocompatibility of nano-sized ceramic particles with several cells types. Though these particles have less than 100 nm in diameter, they act as submicron-sized particles in saline by aggregation that was estimated using laser diffraction particle size analysis (LDS). they act as submicro-sized particles in saline by aggregation based on laser diffraction particle size analysis (LDS). Several types of cells (osteoblasts, osteosarcoma and hepatocyte cells) were exposed to these particles and their cytocompatibility was estimated. Not only the cytotoxic assay but also their static and dynamic morphology under nanoparticles exposure were investigated. The intercellular uptake of particles was determined using a confocal fluorescence microscope. The particles used in this study did not inhibit cellular activity or growth even when their concentrations were high. Only copper oxide particles caused acute cytotoxicity depending on the particle size. The cytotoxicity assay, dynamic behavior of the nanoparticle-exposed cells and their examination under a confocal fluorescence microscope suggests that the irritative reaction was induced by contact between the cells and particles, whereas eluted copper ions are not dominant factor. These results indicate that nano-sized particles used in this study have excellent biocompatibility except copper oxide ones.
我们评估了纳米级陶瓷颗粒与几种细胞类型的生物相容性。尽管这些颗粒的直径小于100纳米，但通过激光衍射粒度分析（LDS）估计，它们在盐溶液中会通过聚集形成亚微米级颗粒。基于激光衍射粒度分析（LDS），它们在盐溶液中通过聚集形成亚微米级颗粒。将几种类型的细胞（成骨细胞、骨肉瘤细胞和肝细胞）暴露于这些颗粒中，并评估它们的细胞相容性。不仅研究了细胞毒性测定，还研究了纳米颗粒暴露下细胞的静态和动态形态。使用共聚焦荧光显微镜测定颗粒的细胞内摄取。本研究中使用的颗粒即使在高浓度下也不会抑制细胞活性或生长。只有氧化铜颗粒会根据颗粒大小引起急性细胞毒性。细胞毒性测定、纳米颗粒暴露细胞的动态行为以及在共聚焦荧光显微镜下的观察表明，细胞与颗粒之间的接触会引发刺激反应，而洗脱的铜离子并非主要因素。这些结果表明，本研究中使用的纳米颗粒除氧化铜颗粒外具有优异的生物相容性。
