Seitoku Eri, Abe Shigeaki, Kusaka Teruo, Nakamura Mariko, Inoue Satoshi, Yoshida Yasuhiro, Sano Hidehiko
J Nanosci Nanotechnol. 2016 Apr;16(4):3373-7. doi: 10.1166/jnn.2016.12309.
In this study, we investigated the behaviors and cytocompatibility response of human cervical carcinoma (HeLa) cells expose to nano-sized particles. Cultivated cells exposed to titanium oxide and indium oxide nanoparticles remained highly viable. In the presence of copper oxide (CuO); however, the cells became seriously inflamed. To understand the mechanism by which CuO causes cell death, we evaluated cell death and apoptosis cytometry. CuO induced cells apoptosis more strongly than exposure to titania nanoparticles. Confocal fluorescence microscopy revealed that the nano-sized particles penetrate the cells.
在本研究中，我们调查了暴露于纳米颗粒的人宫颈癌（HeLa）细胞的行为和细胞相容性反应。暴露于氧化钛和氧化铟纳米颗粒的培养细胞仍具有很高的活力。然而，在存在氧化铜（CuO）的情况下，细胞出现严重炎症。为了了解CuO导致细胞死亡的机制，我们评估了细胞死亡和凋亡细胞计数法。与暴露于二氧化钛纳米颗粒相比，CuO诱导细胞凋亡的作用更强。共聚焦荧光显微镜显示纳米颗粒可穿透细胞。
