Cassini Rudi, Scremin Mara, Contiero Barbara, Drago Andrea, Vettorato Christian, Marcer Federica, di Regalbono Antonio Frangipane
1 Department of Animal Medicine, Production and Health, University of Padova, Viale dell'Università, 16-35020 Legnaro (PD), Italy.
2 Entostudio; Viale del lavoro, 66-35020 Ponte San Nicolò (PD), Italy.
J Am Mosq Control Assoc. 2016 Jun;32(2):139-43. doi: 10.2987/moco-32-02-139-143.1.
Ambient insecticides are receiving increasing attention in many developed countries because of their value in reducing mosquito nuisance. As required by the European Union Biocidal Products Regulation 528/2012, these devices require appropriate testing of their efficacy, which is based on estimating the knockdown and mortality rates of free-flying (free) mosquitoes in a test room. However, evaluations using free mosquitoes present many complexities. The performances of 6 alternative methods with mosquitoes held in 2 different cage designs (steel wire and gauze/plastic) with and without an operating fan for air circulation were monitored in a test room through a closed-circuit television system and were compared with the currently recommended method using free mosquitoes. Results for caged mosquitoes without a fan showed a clearly delayed knockdown effect, whereas outcomes for caged mosquitoes with a fan recorded higher mortality at 24 h, compared to free mosquitoes. Among the 6 methods, cages made of gauze and plastic operating with fan wind speed at 2.5-2.8 m/sec was the only method without a significant difference in results for free mosquitoes, and therefore appears as the best alternative to assess knockdown by ambient insecticides accurately.
由于环境杀虫剂在减少蚊虫滋扰方面的价值，它们在许多发达国家正受到越来越多的关注。根据欧盟生物杀灭剂产品法规528/2012的要求，这些装置需要对其功效进行适当测试，这是基于估计测试室内自由飞行（未关在笼子里）蚊子的击倒率和死亡率。然而，使用自由飞行蚊子进行评估存在许多复杂之处。通过闭路电视系统在测试室内监测了6种替代方法的性能，这些方法使用了2种不同笼子设计（钢丝笼和纱布/塑料笼）饲养的蚊子，且有无运行风扇进行空气循环，并与目前推荐的使用自由飞行蚊子的方法进行了比较。没有风扇的笼养蚊子的结果显示击倒效果明显延迟，而有风扇的笼养蚊子在24小时时的死亡率高于自由飞行的蚊子。在这6种方法中，由纱布和塑料制成的笼子，在风扇风速为2.5 - 2.8米/秒的情况下运行，是唯一一种与自由飞行蚊子的结果没有显著差异的方法，因此似乎是准确评估环境杀虫剂击倒效果的最佳替代方法。
