咖啡因对线虫秀丽隐杆线虫味觉可塑性的抑制作用。
Inhibitory effects of caffeine on gustatory plasticity in the nematode Caenorhabditis elegans.
作者信息
Urushihata Takuya, Takuwa Hiroyuki, Higuchi Yukako, Sakata Kazumi, Wakabayashi Tokumitsu, Nishino Asuka, Matsuura Tetsuya
机构信息
a Division of Thermo-Biosystem Relations , United Graduate School of Agricultural Science, Iwate University , Morioka , Japan.
b Department of Biophysics , Molecular Imaging Center, National Institute of Radiological Sciences , Chiba , Japan.
出版信息
Biosci Biotechnol Biochem. 2016 Oct;80(10):1990-4. doi: 10.1080/09168451.2016.1191327. Epub 2016 Jun 7.
The effects of caffeine on salt chemotaxis learning were investigated using the nematode Caenorhabditis elegans. To estimate the degree of salt chemotaxis learning, nematodes were placed in a mixed solution of NaCl and caffeine, and then the chemotaxis index of NaCl was obtained from the nematodes placed on agar medium after pre-exposure to caffeine concentrations of 0.01, 0.1, 0.3, and 1.0%. Locomotor activity and preference behavior for caffeine were also estimated under these caffeine conditions. Nematodes pre-exposed to 0.3% caffeine showed inhibition of salt chemotaxis learning. Additional experiments indicated that nematodes showed a preference response to the middle concentration of caffeine (0.1%), with preference behavior declining in the 0.3% caffeine condition. Stable locomotor activity was observed under 0.01-0.3% caffeine conditions. These results suggest that salt chemotaxis learning with 0.3% caffeine is useful for investigating the effects of caffeine on learning in nematodes.
利用秀丽隐杆线虫研究了咖啡因对盐趋化性学习的影响。为了评估盐趋化性学习的程度，将线虫置于氯化钠和咖啡因的混合溶液中，然后在预先暴露于浓度为0.01%、0.1%、0.3%和1.0%的咖啡因后，从置于琼脂培养基上的线虫中获得氯化钠的趋化性指数。在这些咖啡因条件下，还评估了线虫对咖啡因的运动活性和偏好行为。预先暴露于0.3%咖啡因的线虫表现出盐趋化性学习受到抑制。额外的实验表明，线虫对中等浓度的咖啡因（0.1%）表现出偏好反应，在0.3%咖啡因条件下偏好行为下降。在0.01 - 0.3%咖啡因条件下观察到稳定的运动活性。这些结果表明，0.3%咖啡因的盐趋化性学习有助于研究咖啡因对线虫学习的影响。
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