Wijayanti L, Fujioka S, Kobayashi M, Sakurai A
a The Institute of Physical and Chemical Research (RIKEN) , Wako-shi, Saitama 351-01 , Japan.
Biosci Biotechnol Biochem. 1996 Jan;60(5):852-5. doi: 10.1271/bbb.60.852.
The role of endogenous gibberellin (GA) in the flowering of the short-day plant, Pharbitis nil, was investigated by using uniconazole, which is a specific inhibitor of GA biosynthesis. Both the endogenous GA level and flowering response decreased with increasing concentration of uniconazole applied via the roots. The strongest inhibition of flowering was observed when uniconazole was applied one day before a 15-h dark treatment. The inhibition by uniconazole was overcome by an application of GAs to the plumules, the order of effectiveness of the endogenous GAs in P. nil being GA1 ≧GA20>GA19≧GA44>GA53»GAH. This is the first report of the correlation between the endogenous GA level and flowering response in P. nil. It was found that endogenous GAs were required for the flowering of P. nil during or just after the dark period.
通过使用烯效唑（一种赤霉素生物合成的特异性抑制剂），对内源赤霉素（GA）在短日植物牵牛花开花过程中的作用进行了研究。随着通过根部施用的烯效唑浓度增加，内源GA水平和开花反应均降低。当在15小时黑暗处理前一天施用烯效唑时，观察到对开花的抑制作用最强。通过向子叶施用赤霉素可克服烯效唑的抑制作用，牵牛花中内源赤霉素的有效性顺序为GA1≧GA20>GA19≧GA44>GA53»GAH。这是关于牵牛花内源GA水平与开花反应之间相关性的首次报道。研究发现，在黑暗期或黑暗期刚结束时，牵牛花开花需要内源赤霉素。
