Hou Weizhou, Thorne Steve H
Department of Radiology, Hillman Cancer Center, University of Pittsburgh, Suite 1.46e, 5117 Centre Avenue, Pittsburgh, PA, 15213, USA.
Methods Mol Biol. 2016;1444:67-72. doi: 10.1007/978-1-4939-3721-9_7.
The capacity to combine noninvasive whole animal imaging of genetic reporters and exogenously added probes in a single animal makes fluorescence imaging a powerful tool for investigating molecular events in live animals in preclinical research. However, the adsorption and diffraction properties of light passing through tissues mean that the choice of reporters, models, and imaging systems needs to be carefully considered. Here, we describe approaches to design and run experiments incorporating noninvasive whole animal fluorescence imaging into small animal imaging studies.
在单个动物中结合基因报告分子的非侵入性全动物成像与外源性添加探针的能力，使荧光成像成为临床前研究中用于研究活体动物分子事件的强大工具。然而，光穿过组织时的吸收和衍射特性意味着需要仔细考虑报告分子、模型和成像系统的选择。在此，我们描述了将非侵入性全动物荧光成像纳入小动物成像研究的实验设计和实施方法。
