使用碳纳米管在第二近红外窗口进行的体内荧光成像。
In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes.
作者信息
Hong Guosong, Dai Hongjie
机构信息
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA, 02138, USA.
Department of Chemistry, Stanford University, Stanford, CA, 94305, USA.
出版信息
Methods Mol Biol. 2016;1444:167-81. doi: 10.1007/978-1-4939-3721-9_15.
In vivo fluorescence imaging in the second near-infrared window (NIR-II window, 1000-1700 nm) is a powerful imaging technique that emerged in recent years. This imaging tool allows for noninvasive, deep-tissue visualization and interrogation of anatomical features and functions with improved imaging resolution and contrast at greater tissue penetration depths than traditional fluorescence imaging. Here, we present the detailed protocol for conducting NIR-II fluorescence imaging in live animals, including the procedures for preparation of biocompatible and NIR-II fluorescent carbon nanotube solution, live animal administration and NIR-II fluorescence image acquisition.
在第二近红外窗口（NIR-II窗口，1000 - 1700纳米）进行的体内荧光成像，是近年来出现的一种强大的成像技术。这种成像工具能够实现非侵入性的、对深层组织的可视化以及对解剖特征和功能的探究，与传统荧光成像相比，在更大的组织穿透深度下具有更高的成像分辨率和对比度。在此，我们展示了在活体动物中进行NIR-II荧光成像的详细方案，包括生物相容性NIR-II荧光碳纳米管溶液的制备、活体动物给药以及NIR-II荧光图像采集的步骤。
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