Rodríguez-Cerezo E, García-Arenal F
Departamento de Patología Vegetal, E.T.S.I. Argrónomos, Madrid, Spain.
Virology. 1989 Jun;170(2):418-23. doi: 10.1016/0042-6822(89)90432-7.
The genetic heterogeneity in a population of the U5 strain of tobacco mosaic virus (U5-TMV) was studied. The T1 fingerprint characterizing a cloned population did not vary after a new cloning step in the local lesion host Nicotiana tabacum Xanthi-nc, nor during four series of 20 passages in the systemic host N. tabacum Samsum. No heterogeneity was observed among 10 clones derived from the cloned populations, while 1 of 18 clones derived from a 20-fold passaged population differed from the rest in 1 of 55 oligonucleotides. A higher heterogeneity was found in an uncloned field isolate in which 2 of 10 clones differed in type in 1 and 2 oligonucleotides, respectively. These data agree with those reported for bacterial and animal RNA viruses and are compatible with the quasi-species model for RNA populations. On the other hand, the intrapopulational heterogeneities found for U5-TMV are considerably smaller than those reported for other RNA viruses, our data showing a high genetic stability for U5-TMV.
对烟草花叶病毒U5株系（U5-TMV）群体中的遗传异质性进行了研究。表征克隆群体的T1指纹图谱在局部病斑寄主烟草品种Xanthi-nc中进行新的克隆步骤后，以及在系统寄主烟草品种Samsum中进行的4组每组20次传代过程中均未发生变化。从克隆群体衍生的10个克隆中未观察到异质性，而从传代20倍的群体衍生的18个克隆中有1个在55个寡核苷酸中的1个与其他克隆不同。在未克隆的田间分离物中发现了更高的异质性，其中10个克隆中有2个分别在1个和2个寡核苷酸的类型上存在差异。这些数据与针对细菌和动物RNA病毒报道的数据一致，并且与RNA群体的准种模型相符。另一方面，U5-TMV群体内的异质性比其他RNA病毒报道的要小得多，我们的数据表明U5-TMV具有高度的遗传稳定性。
