Liu Wen-Yue, Wu X U, Liao Cheng-Quan, Shen Jie, Li Jun
Department of Thoracic and Cardiovascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China; Department of Thoracic Surgery, Yuebei People's Hospital, Shaoguan, Guangdong 512026, P.R. China.
Department of Thoracic and Cardiovascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.
Oncol Lett. 2016 Jun;11(6):3681-3685. doi: 10.3892/ol.2016.4437. Epub 2016 Apr 14.
Despite extensive investigations of therapeutic improvements for surgical techniques, chemotherapy and chemoradiotherapy, esophageal squamous cell carcinoma (ESCC) remains one of the most aggressive forms of cancer, and the prognosis for patients with advanced ESCC remains poor. Therefore, effective therapies are urgently required in order to improve the prognosis of patients with ESCC. TE-1 cells were treated with gambogic acid (GA), and then subjected to western blot analysis, TUNEL assay and caspase activity analysis. GA significantly induced apoptosis in ESCC TE-1 cells. In addition, the antitumor activity of GA was accompanied by the decreased expression of phosphorylated-protein kinase B (p-AKT) and nuclear factor of κ light polypeptide gene enhancer in B-cells 1 (NF-κB). The inhibition of protein kinase B (AKT) and NF-κB activation by chemical inhibitors augmented the apoptotic effect responses to GA in the TE-1 cells. The pan-caspase inhibitor z-VAD-fmk (zVAD) decreased GA-induced apoptosis. Furthermore, zVAD attenuated GA-induced growth inhibition in TE-1 cells. GA induced apoptosis in ESCC TE-1 via suppression of NF-κB pathway. The findings of the present study may provide a novel insight into ESCC treatment.
尽管对外科手术技术、化疗及放化疗的治疗改进进行了广泛研究，但食管鳞状细胞癌（ESCC）仍然是最具侵袭性的癌症形式之一，晚期ESCC患者的预后仍然很差。因此，迫切需要有效的治疗方法来改善ESCC患者的预后。用藤黄酸（GA）处理TE-1细胞，然后进行蛋白质印迹分析、TUNEL检测和半胱天冬酶活性分析。GA显著诱导ESCC TE-1细胞凋亡。此外，GA的抗肿瘤活性伴随着磷酸化蛋白激酶B（p-AKT）和B细胞κ轻链多肽基因增强子核因子1（NF-κB）表达的降低。化学抑制剂对蛋白激酶B（AKT）和NF-κB激活的抑制增强了TE-1细胞对GA的凋亡效应反应。泛半胱天冬酶抑制剂z-VAD-fmk（zVAD）降低了GA诱导的凋亡。此外，zVAD减弱了GA对TE-1细胞生长的抑制作用。GA通过抑制NF-κB途径诱导ESCC TE-1细胞凋亡。本研究结果可能为ESCC治疗提供新的见解。
