Skalicka-Woźniak Krystyna, Mroczek Tomasz, Walasek Magdalena, Głowniak Kazimierz
Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, Lublin, Poland.
Planta Med. 2016 Jul;82(11-12):1105-9. doi: 10.1055/s-0042-108738. Epub 2016 Jun 10.
For the first time, the separation of coumarin derivatives from the petroleum ether extract of the fruits of Mutellina purpurea through high-performance countercurrent chromatography (HPCCC) is described. Four compounds, pteryxin (1; 2.72 mg), hyuganin C (2; 7.94 mg), osthol (3; 4.30 mg), and hyuganin A (4; 3.09 mg), were obtained in a single run following the injection of crude extract (300 mg). Additionally, auraptenol (5) and hyuganin D (6) were identified using LC-ESI-(Q)TOF-MS. The structures of the isolated compounds were elucidated through spectroscopic (NMR and MS) methods. This is apparently the first report of the identification of dihydropyranocoumarins in this plant, and the first time that HPCCC was used to separate them.
首次描述了通过高效逆流色谱法（HPCCC）从紫背天葵果实的石油醚提取物中分离香豆素衍生物。在注入粗提取物（300mg）后，单次运行就获得了四种化合物，分别是翼藜素（1；2.72mg）、补骨脂宁C（2；7.94mg）、蛇床子素（3；4.30mg）和补骨脂宁A（4；3.09mg）。此外，使用液相色谱-电喷雾电离-（四极杆）飞行时间质谱法（LC-ESI-(Q)TOF-MS）鉴定了金合欢醇（5）和补骨脂宁D（6）。通过光谱（核磁共振和质谱）方法阐明了分离出的化合物的结构。这显然是该植物中二氢吡喃香豆素鉴定的首次报道，也是首次使用HPCCC对其进行分离。
