Goto A, Kunioka M
a Mitsubishi Paper Mills Limited, Tsukuba Research Laboratories , Wadai, Tsukuba-shi , Ibaraki 300-42 , Japan.
b Research Institute for Polymers and Textiles , Higashi, Tsukuba-shi , Ibaraki 305 , Japan.
Biosci Biotechnol Biochem. 1992 Jan;56(7):1031-5. doi: 10.1271/bbb.56.1031.
Poly(γ-glutamic acid) (PGA) production in Bacillus subtilis IF03335 was studied. When citric acid as a carbon source was added to a glutamic acid medium containing L-glutamic acid and ammonium sulfate, a large amount of pure PGA was produced. On the other hand, when glucose was added to the glutamic acid medium, a by-product was produced, which seemed to be a polysaccharide. Moreover, the mode of hydrolysis was investigated with PGA in aqueous solutions at 80, 100, and 120°C by monitoring the time-dependent changes in the molecular weights. Hydrolytic degradation of PGA was found to proceed through a random chain scission.
研究了枯草芽孢杆菌IF03335中聚（γ-谷氨酸）（PGA）的生产情况。当向含有L-谷氨酸和硫酸铵的谷氨酸培养基中添加柠檬酸作为碳源时，会产生大量的纯PGA。另一方面，当向谷氨酸培养基中添加葡萄糖时，会产生一种副产物，它似乎是一种多糖。此外，通过监测80、100和120°C水溶液中PGA分子量随时间的变化，研究了其水解方式。发现PGA的水解降解是通过随机链断裂进行的。
