Arutiunian L A, Batikian G G, Simoniants A A
Zh Evol Biokhim Fiziol. 1989 Jan-Feb;25(1):10-4.
Using disc electrophoresis in polyacrylamide gel, studies have been made on the content of isoenzymes of lactate dehydrogenase in the mitochondria and cytoplasm from the brain, heart and liver after thyroxine administration to embryonal, early postnatal and adult hens. After this administration, the isoenzymic composition becomes more complex, redistribution of separate isozymes being observed in ontogenesis. The level of aerobic form of LDH increases at early stages of ontogenesis, that of anaerobic ones--in adult birds. Basing on their different functional role due to the presence of H- and M-subunits, it may be suggested that thyroxine administration results in a compensatory increase of the oxidative or glycolytic processes at the corresponding stages of ontogenesis, these changes reflecting phylogenetic peculiarities of the formation of the hormonal control of lactate dehydrogenase in tissues of birds.
利用聚丙烯酰胺凝胶圆盘电泳技术，对给胚胎期、出生后早期及成年母鸡注射甲状腺素后，其脑、心脏和肝脏线粒体及细胞质中乳酸脱氢酶同工酶的含量进行了研究。注射甲状腺素后，同工酶组成变得更加复杂，在个体发育过程中观察到不同同工酶的重新分布。有氧形式的乳酸脱氢酶水平在个体发育早期升高，无氧形式的乳酸脱氢酶水平在成年鸟类中升高。基于其由于H亚基和M亚基的存在而具有的不同功能作用，可以认为注射甲状腺素会导致个体发育相应阶段氧化或糖酵解过程的代偿性增加，这些变化反映了鸟类组织中乳酸脱氢酶激素调控形成的系统发育特性。
