Švachová Veronika, Vojtová Lucy, Pavliňák David, Vojtek Libor, Sedláková Veronika, Hyršl Pavel, Alberti Milan, Jaroš Josef, Hampl Aleš, Jančář Josef
Institute of Materials Chemistry, Brno University of Technology, Czech Republic.
CEITEC - Central European Institute of Technology, Brno University of Technology, Czech Republic; SCITEG, a.s., Brno, Czech Republic.
Mater Sci Eng C Mater Biol Appl. 2016 Oct 1;67:493-501. doi: 10.1016/j.msec.2016.05.059. Epub 2016 May 16.
Novel hydrolytically stable gelatin nanofibers modified with sodium or calcium salt of oxycellulose were prepared by electrospinning method. The unique inhibitory effect of these nanofibers against Escherichia coli bacteria was examined by luminometric method. Biocompatibility of these gelatin/oxycellulose nanofibers with eukaryotic cells was tested using human lung adenocarcinoma cell line NCI-H441. Cells firmly adhered to nanofiber surface, as determined by scanning electron microscopy, and no signs of cell dying were detected by fluorescent live/dead assay. We propose that the newly developed gelatin/oxycellulose nanofibers could be used as promising scaffold for lung disease modeling and anti-cancer drug testing.
通过静电纺丝法制备了用氧化纤维素钠盐或钙盐改性的新型水解稳定明胶纳米纤维。采用发光法研究了这些纳米纤维对大肠杆菌的独特抑制作用。使用人肺腺癌细胞系NCI-H441测试了这些明胶/氧化纤维素纳米纤维与真核细胞的生物相容性。通过扫描电子显微镜测定，细胞牢固地附着在纳米纤维表面，并且通过荧光活/死测定未检测到细胞死亡的迹象。我们认为，新开发的明胶/氧化纤维素纳米纤维有望用作肺病建模和抗癌药物测试的支架。
