School of Biotechnology and Health Sciences , Wuyi University , Jiangmen , Guangdong 529020 , People's Republic of China.
School of Mechanical and Electric Engineering , Guangzhou University , Guangzhou , Guangdong 510006 , People's Republic of China.
J Agric Food Chem. 2019 Feb 27;67(8):2227-2234. doi: 10.1021/acs.jafc.8b06226. Epub 2019 Feb 15.
Natural and edible materials have attracted increasing attention in food packaging, which could overcome the serious environmental issues caused by conventional non-biodegradable synthetic packaging. In this work, gelatin nanofibers incorporated with two kinds of essential oil (EO), peppermint essential oil (PO) and chamomile essential oil (CO), were fabricated by electrospinning for potential edible packaging application. Electron microscopy showed that smooth and uniform morphology of the gelatin/EOs was obtained, and the diameter of nanofibers was mostly enlarged with the increase of the EO content. The proton nuclear magnetic resonance spectrum confirmed the existence of PO and CO in nanofibers after electrospinning. The addition of EOs led to an enhancement of the water contact angle of nanofibers. The antioxidant activity was significantly improved for the nanofibers loaded with CO, while the antibacteria activity against Escherichia coli and Staphylococcus aureus was better for the fibers with PO addition. The combination of half PO and half CO in nanofibers compensated for their respective limitations and exhibited optimum bioactivities. Finally, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay with NIH-3T3 fibroblasts demonstrated the absence of cytotoxicity of the gelatin/EO nanofibers. Thus, our studies suggest that the developed gelatin/PO/CO nanofiber could be a promising candidate for edible packaging.
天然可食用材料在食品包装中受到越来越多的关注，因为它们可以克服传统不可生物降解的合成包装所带来的严重环境问题。在这项工作中，通过静电纺丝制备了两种精油（薄荷油和洋甘菊油）掺入明胶纳米纤维的复合纳米纤维，用于潜在的可食用包装应用。电子显微镜显示，明胶/精油具有光滑且均匀的形态，并且纳米纤维的直径随着精油含量的增加而显著增大。质子核磁共振谱证实了静电纺丝后纳米纤维中存在薄荷油和洋甘菊油。精油的添加导致纳米纤维的水接触角增大。载有 CO 的纳米纤维的抗氧化活性显著提高，而添加 PO 的纤维对大肠杆菌和金黄色葡萄球菌的抗菌活性更好。纳米纤维中半量的 PO 和半量的 CO 的结合弥补了它们各自的局限性，并表现出最佳的生物活性。最后，用 NIH-3T3 成纤维细胞进行的 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐试验表明，明胶/精油纳米纤维没有细胞毒性。因此，我们的研究表明，开发的明胶/PO/CO 纳米纤维可能是一种有前途的可食用包装材料。
