Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Cidade Universitária, CCS - Bl. I, Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil.
Braz J Microbiol. 2020 Jun;51(2):585-591. doi: 10.1007/s42770-019-00166-3. Epub 2019 Oct 30.
The human polyomaviruses (HPyVs) 10 and 11 have been detected in faecal material and are tentatively associated with diarrhoeal disease. However, to date, there are insufficient data to confirm or rule out this association, or even to provide basic information about these viruses, such as how they are distributed in the population, the persistence sites and their pathogenesis. In this study, we analysed stool specimens from Brazilian children with and without acute diarrhoea to investigate the excretion of HPyV10 and HPyV11 as well as their possible associations with diarrhoea. A total of 460 stool specimens were obtained from children with acute diarrhoea of unknown aetiology, and 106 stool specimens were obtained from healthy asymptomatic children under 10 years old. Samples were collected during the periods of 1999-2006, 2010-2012 and 2016-2017, and found previously to be negative for other enteric viruses and bacteria. The specimens were screened for HPyV10 and HPyV11 DNA by the polymerase chain reaction (PCR). Randomly selected positive samples were sequenced to confirm the presence of HPyV10 and HPyV11. The sequenced strains showed a percent of nucleotide identity of 93.4-99.6% and 85.5-98.9% with the reference HPyV10 and HPyV11 strains, respectively, confirming the PCR results. HPyV10 and HPyV11 were detected in 7.2% and 4.7% of the stool specimens from children with and without diarrhoea, respectively. The prevalence of both viruses was the same among children with diarrhoea and healthy children. There was also no difference between boys and girls or the degree of disease (severe, moderate or mild) among groups. Phylogenetic analysis showed that all of the genotypes described so far for HPyV10 and HPyV11 circulate in Rio de Janeiro. Our results do not support an association between HPyV10 and HPyV11 in stool samples and paediatric gastroenteritis. Nevertheless, the excretion of HPyV10 and HPyV11 in faeces indicates that faecal-oral transmission is possible.
人多瘤病毒（HPyV）10 和 11 已在粪便中检测到，与腹泻病有暂定关联。然而，迄今为止，尚无足够的数据来证实或排除这种关联，甚至无法提供有关这些病毒的基本信息，例如它们在人群中的分布、持续存在的部位及其发病机制。在这项研究中，我们分析了巴西患有和不患有急性腹泻的儿童的粪便标本，以研究 HPyV10 和 HPyV11 的排泄情况及其与腹泻的可能关联。共从患有病因不明的急性腹泻的儿童中获得 460 份粪便标本，从 10 岁以下无症状健康儿童中获得 106 份粪便标本。这些样本采集于 1999-2006 年、2010-2012 年和 2016-2017 年期间，此前发现这些样本均为其他肠道病毒和细菌阴性。通过聚合酶链反应（PCR）对样本进行 HPyV10 和 HPyV11 DNA 的筛查。随机选择阳性样本进行测序以确认 HPyV10 和 HPyV11 的存在。测序株与参考 HPyV10 和 HPyV11 株的核苷酸同一性分别为 93.4-99.6%和 85.5-98.9%，证实了 PCR 结果。患有和不患有腹泻的儿童的粪便标本中分别检测到 7.2%和 4.7%的 HPyV10 和 HPyV11。两种病毒在腹泻儿童和健康儿童中的流行率相同。在男孩和女孩之间以及疾病程度（严重、中度或轻度）之间也没有差异。系统发育分析显示，迄今为止描述的所有 HPyV10 和 HPyV11 基因型都在里约热内卢流行。我们的结果不支持粪便样本中 HPyV10 和 HPyV11 与小儿肠炎之间的关联。然而，HPyV10 和 HPyV11 在粪便中的排泄表明粪-口传播是可能的。
