Kataoka Saori, Mukai Yuuka, Takebayashi Mihoko, Kudo Megumi, Acuram Uson Rachael, Kurasaki Masaaki, Sato Shin
Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori 030-8505, Japan.
School of Nutrition and Dietetics, Faculty of Health and Social Work, Kanagawa University of Human Services, Kanagawa 238-8522, Japan.
Reprod Biol. 2016 Jun;16(2):165-73. doi: 10.1016/j.repbio.2016.01.002. Epub 2016 Feb 22.
Excessive maternal fructose intake during pregnancy and in early postnatal life has metabolic consequences for the offspring. We investigated the effects of melinjo (Gnetum gnemon) extract (MeE) intake during lactation on the expression and phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) in the liver of offspring from excessive fructose-fed pregnant dams. Pregnant Wistar rats received a normal diet and 100g/L fructose solution during gestation ad libitum. At delivery, dams were divided into two groups: a control diet (FC) or a 0.1% MeE-containing diet (FM) fed during lactation. The dams that were not treated with fructose were fed a control diet (CC). At postnatal week 3, some pups were sacrificed, while the remaining continued to receive a normal diet and were sacrificed at week 17. Blood chemistry and phosphorylation levels of AMPK and acetyl-coenzyme A carboxylase (ACC) were evaluated. Plasma glucose levels in FC female offspring increased compared to that receiving CC at weeks 3 and 17; however, the levels in FM female offspring decreased at week 17. The insulin levels in FM female offspring decreased significantly compared to that in FC female offspring at week 3. Hepatic AMPK phosphorylation was upregulated in FM offspring at week 3 and in female, but not male, offspring at week 17. ACC phosphorylation in FM female offspring was upregulated at week 17. Our results suggest that maternal MeE intake during lactation may modulate the hepatic AMPK pathways in female offspring.
孕期及产后早期母体过量摄入果糖会对后代产生代谢影响。我们研究了哺乳期摄入美藤果（Gnetum gnemon）提取物（MeE）对过量喂食果糖的怀孕母鼠所产后代肝脏中腺苷单磷酸激活蛋白激酶（AMPK）表达和磷酸化的影响。怀孕的Wistar大鼠在妊娠期随意摄入正常饮食和100g/L果糖溶液。分娩时，母鼠分为两组：哺乳期喂食对照饮食（FC）或含0.1%MeE的饮食（FM）。未用果糖处理的母鼠喂食对照饮食（CC）。出生后第3周，处死部分幼崽，其余继续喂食正常饮食，并在第17周处死。评估血液生化指标以及AMPK和乙酰辅酶A羧化酶（ACC）的磷酸化水平。与CC组相比，FC组雌性后代在第3周和第17周时血浆葡萄糖水平升高；然而，FM组雌性后代在第17周时葡萄糖水平下降。在第3周时，FM组雌性后代的胰岛素水平与FC组雌性后代相比显著降低。在第3周时，FM组后代肝脏中AMPK磷酸化上调，在第17周时，雌性后代（而非雄性后代）肝脏中AMPK磷酸化上调。在第17周时，FM组雌性后代ACC磷酸化上调。我们的结果表明，哺乳期母体摄入MeE可能会调节雌性后代肝脏中的AMPK通路。
