Department of Biological Science and Technology, School of Environmental Studies & State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences (Wuhan), Wuhan 430074, People's Republic of China.
Department of Biological Science and Technology, School of Environmental Studies & State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences (Wuhan), Wuhan 430074, People's Republic of China.
Water Res. 2016 Sep 15;101:393-401. doi: 10.1016/j.watres.2016.05.058. Epub 2016 May 20.
A population of arsenite-oxidizing microorganisms enriched from the tailing of the Shimen realgar mine was used to generate biofilms on the surfaces of perlites. This bioreactor is able to completely oxidize 1100 μg/L As(III) dissolved in simulated groundwater into As(V) within 10 min; after 140 days of operation, approximately 20 min were required to completely oxidize the same concentration of As(III). Analysis for the 16S rRNA genes of the microbial community showed that Bacteroidetes and Proteobacteria are dominant in the reactor. Six different bacterial strains were randomly isolated from the reactor. Function and gene analysis indicated that all the isolates possess arsenite-oxidizing activity, and five of them are chemoautotrophic. Further analysis showed that a large diversity of AioAs and two types of RuBisCOs are present in the microbial community. This suggests that many chemoautotrophic arsenite-oxidizing microorganisms were responsible for quick oxidation of arsenite in the reactor. We also found that the reactor is easily regenerated and its number is readily expanded. To the best of our knowledge, the arsenite-oxidizing efficiency, which was expressed as the minimum time for complete oxidization of a certain concentration of As(III) under a single operation, of this bioreactor is the highest among the described bioreactors; it is also the most stable, economic and environment-friendly.
从石门雄黄矿尾矿中富集的亚砷酸盐氧化微生物种群被用于在珍珠岩表面生成生物膜。该生物反应器能够在 10 分钟内将模拟地下水中溶解的 1100μg/L 的 As(III)完全氧化成 As(V)；经过 140 天的运行，完全氧化相同浓度的 As(III)只需要大约 20 分钟。对微生物群落的 16S rRNA 基因分析表明，反应器中优势菌为拟杆菌门和变形菌门。从反应器中随机分离出 6 种不同的细菌菌株。功能和基因分析表明，所有分离株都具有亚砷酸盐氧化活性，其中 5 株为化能自养菌。进一步分析表明，微生物群落中存在大量的 AioAs 和两种类型的 RuBisCO。这表明许多化能自养的亚砷酸盐氧化微生物是导致反应器中亚砷酸盐快速氧化的原因。我们还发现，该反应器易于再生，并且易于扩展其数量。据我们所知，该生物反应器的砷酸盐氧化效率（以单个运行中完全氧化某一浓度的 As(III)所需的最短时间来表示）在已描述的生物反应器中是最高的；它也是最稳定、经济和环保的。
