State Key Laboratory of Biogeology and Environmental Geology & School of Environmental Studies, China University of Geosciences (Wuhan), 430074, Wuhan, People's Republic of China.
Ecotoxicology. 2021 Sep;30(7):1437-1445. doi: 10.1007/s10646-020-02283-4. Epub 2020 Oct 11.
Arsenite (As(III)) was considered to be of great concern in acid mine drainage (AMD). A promising approach for cleaning up of arsenite from AMD is microbial oxidation of As(III) followed by adsorptions. However, there is virtually no research about the acidophilic bioreactor for As(III) oxidation so far. In this study, we formed a new biofilm bioreactor with a consortium of acidophilic As(III) oxidation bacteria. It is totally chemoautotrophic, with no need to add any carbon or other materials during the operations. It works well under pH 3.0-4.0, capable of oxidizing 1.0-20.0 mg/L As(III) in 3.0-4.5 h, respectively. A continuous operation of the bioreactor suggests that it is very stable and sustainable. Functional gene detection indicated that the biofilms possessed a unique diversity of As(III) oxidase genes. Taken together, this acidophilic bioreactor has great potential for industrial applications in the cleaning up of As(III) from AMD solution.
亚砷酸盐（As(III)）被认为是酸性矿山排水（AMD）中非常值得关注的物质。微生物氧化 As(III) 随后进行吸附是从 AMD 中去除亚砷酸盐的一种很有前途的方法。然而，迄今为止，实际上没有关于用于 As(III)氧化的嗜酸生物反应器的研究。在这项研究中，我们用嗜酸亚砷酸盐氧化细菌的混合物形成了一种新型生物膜生物反应器。它完全是化能自养的，在操作过程中不需要添加任何碳或其他物质。它在 pH 值为 3.0-4.0 下运行良好，分别能够在 3.0-4.5 小时内氧化 1.0-20.0mg/L 的 As(III)。生物反应器的连续运行表明它非常稳定和可持续。功能基因检测表明，生物膜具有独特的多样性的亚砷酸盐氧化酶基因。总的来说，这种嗜酸生物反应器在从 AMD 溶液中去除 As(III)方面具有很大的工业应用潜力。
