Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, PR China.
Bioresour Technol. 2016 Sep;216:699-705. doi: 10.1016/j.biortech.2016.06.001. Epub 2016 Jun 2.
Using microbial community MC1 to pretreat lignocellulosic materials increased the yield of biogas production, and the substrate did not need to be sterilized, lowering the cost. Rotted silage maize straw carries many microbes. To determine whether such contamination affects MC1, rotted silage maize straw was pretreated with MC1 prior to biogas production. The decreases in the weights of unsterilized and sterilized rotted silage maize straw were similar, as were their carboxymethyl cellulase activities. After 5d pretreatment, denaturing gradient gel electrophoresis and quantitative polymerase chain reaction results indicated that the proportions of five key strains in MC1 were the same in the unsterilized and sterilized groups; thus, MC1 was resistant to microbial contamination. However, its resistance to contamination decreased as the degradation time increased. Following pretreatment, volatile fatty acids, especially acetic acid, were detected, and MC1 enhanced biogas yields by 74.7% compared with the untreated group.
利用微生物群落 MC1 预处理木质纤维素材料可以提高沼气产量，并且不需要对底物进行灭菌，从而降低了成本。腐烂青贮玉米秸秆携带许多微生物。为了确定这种污染是否会影响 MC1，在进行沼气生产之前，用 MC1 预处理腐烂青贮玉米秸秆。未灭菌和灭菌的腐烂青贮玉米秸秆的重量减少相似，其羧甲基纤维素酶活性也相似。预处理 5d 后，变性梯度凝胶电泳和定量聚合酶链反应结果表明，未灭菌组和灭菌组中 MC1 中 5 种关键菌株的比例相同；因此，MC1 对微生物污染具有抗性。然而，随着降解时间的增加，其抗污染能力下降。预处理后，检测到挥发性脂肪酸，特别是乙酸，并且 MC1 使沼气产量比未处理组提高了 74.7%。
