Poszytek Krzysztof, Pyzik Adam, Sobczak Adam, Lipinski Leszek, Sklodowska Aleksandra, Drewniak Lukasz
University of Warsaw, Faculty of Biology, Laboratory of Environmental Pollution Analysis, Miecznikowa 1, 02-096 Warsaw, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
Anaerobe. 2017 Aug;46:46-55. doi: 10.1016/j.anaerobe.2017.02.011. Epub 2017 Feb 17.
The main aim of this study was to evaluate the effect of the source of microorganisms on the selection of hydrolytic consortia dedicated to anaerobic digestion of maize silage. The selection process was investigated based on the analysis of changes in the hydrolytic activity and the diversity of microbial communities derived from (i) a hydrolyzer of a commercial agricultural biogas plant, (ii) cattle slurry and (iii) raw sewage sludge, during a series of 10 passages. Following the selection process, the adapted consortia were thoroughly analyzed for their ability to utilize maize silage and augmentation of anaerobic digestion communities. The results of selection of the consortia showed that every subsequent passage of each consortium leads to their adaptation to degradation of maize silage, which was manifested by the increased hydrolytic activity of the adapted consortia. Biodiversity analysis (based on the 16S rDNA amplicon sequencing) confirmed the changes microbial community of each consortium, and showed that after the last (10th) passage all microbial communities were dominated by the representatives of Lactobacillaceae, Prevotellaceae, Veillonellaceae. The results of the functional analyses showed that the adapted consortia improved the efficiency of maize silage degradation, as indicated by the increase in the concentration of glucose and volatile fatty acids (VFAs), as well as the soluble chemical oxygen demand (sCOD). Moreover, bioaugmentation of anaerobic digestion communities by the adapted hydrolytic consortia increased biogas yield by 10-29%, depending on the origin of the community. The obtained results also indicate that substrate input (not community origin) was the driving force responsible for the changes in the community structure of hydrolytic consortia dedicated to anaerobic digestion.
本研究的主要目的是评估微生物来源对用于玉米青贮厌氧消化的水解菌群选择的影响。基于对来自（i）商业农业沼气厂的水解器、（ii）牛粪浆和（iii）原污水污泥的水解活性变化和微生物群落多样性的分析，研究了一系列10次传代过程中的选择过程。在选择过程之后，对适应后的菌群进行了全面分析，以评估它们利用玉米青贮的能力以及对厌氧消化群落的增强作用。菌群选择结果表明，每个菌群的每一次后续传代都会使其适应玉米青贮的降解，这表现为适应后菌群水解活性的提高。生物多样性分析（基于16S rDNA扩增子测序）证实了每个菌群的微生物群落变化，并表明在最后一次（第10次）传代后，所有微生物群落均以乳杆菌科、普雷沃氏菌科、韦荣氏菌科的代表为主。功能分析结果表明，适应后的菌群提高了玉米青贮的降解效率，表现为葡萄糖、挥发性脂肪酸（VFAs）以及可溶性化学需氧量（sCOD）浓度的增加。此外，适应后的水解菌群对厌氧消化群落的生物强化使沼气产量提高了10%-29%，这取决于群落的来源。所得结果还表明，底物输入（而非群落来源）是导致用于厌氧消化的水解菌群群落结构变化的驱动力。
