Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Folia Microbiol (Praha). 2012 Jul;57(4):281-4. doi: 10.1007/s12223-012-0125-6. Epub 2012 Apr 11.
The qualitative and quantitative changes in the bacterial community composition in two mesophilic, commercially used biogas plants were monitored by denaturing gradient gel electrophoresis (DGGE) and real-time PCR. The main objective was to evaluate the influence of the co-substrate maize silage on total bacteria and some selected bacterial groups by comparing full-scale reactors fed solely with pig manure or additionally with maize silage. DGGE fingerprints reflected shifts in the bacterial community structure associated with maize silage as co-substrate and the real-time PCR results showed clear changes in the quantitative composition of the bacterial consortia of each fermenter. A clear dominance of Clostridia in all surveyed fermenters and considerably lower abundance of Bacteroidetes in the biogas plant fed with maize silage was shown.
通过变性梯度凝胶电泳（DGGE）和实时 PCR 监测了两个中温、商业使用的沼气厂中细菌群落组成的定性和定量变化。主要目的是通过比较仅用猪粪或额外添加青贮玉米作为共底物的全规模反应器，评估共底物青贮玉米对总细菌和一些选定细菌群的影响。DGGE 指纹图谱反映了与共底物青贮玉米相关的细菌群落结构的变化，实时 PCR 结果表明每个发酵罐中细菌联合体的定量组成发生了明显变化。所有调查的发酵罐中以梭菌为主，而青贮玉米作为共底物的沼气厂中拟杆菌的丰度明显较低。
