Weihs Wolfgang, Warenits Alexandra-M, Ettl Florian, Magnet Ingrid A M, Teubenbacher Ursula, Hilpold Andreas, Schober Andreas, Testori Christoph, Tiboldi Akos, Mag Katharina Tillmann, Holzer Michael, Hoegler Sandra, Janata Andreas, Sterz Fritz
Department of Emergency Medicine, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Wien, Austria.
Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Wien, Austria.
BMC Vet Res. 2016 Jun 13;12(1):103. doi: 10.1186/s12917-016-0740-6.
Evaluating beneficial effects of potential protective therapies following cardiac arrest in rodent models could be enhanced by exploring behavior and cognitive functions. The Morris Water Maze is a well-known cognitive paradigm to test spatial learning and memory.
Behavioral testing with the Morris Water Maze in Sprague-Dawley rats (300 ± 25 g) resuscitated after 8 min of ventricular fibrillation cardiac arrest was carried out 5 and 12 weeks after cardiac arrest (CA) and compared to results of naïve rats (CONTROL). At 5 weeks, within each group latency time to reach the hidden platform (reflecting spatial learning) decreased equally from day 1 to 4 (CA: 105.6 ± 8.2 vs. 8.9 ± 1.2 s, p < 0.001;
75.5 ± 13.2 vs. 17.1 ± 4.5, p < 0.001) with no differences between groups (p = 0.138). In the probe trial 24 h after the last trial, time spent in the target sector (reflecting memory recall) within each group was significantly longer (CA: 25 ± 1.3;
24.7 ± 2.5 s) than in each of the three other sectors (CA: 7.7 ± 0.7, 14.3 ± 2.5, 8.4 ± 0.8 and
7.8 ± 1.2, 11.7 ± 1.5, 10.3 ± 1.6 s) but with no significantly differences between groups. Seven days later (reflecting memory retention), control group animals remained significantly longer in the target sector compared to every other sector, whereas the cardiac arrest group animals did not. Even 12 weeks after cardiac arrest, the single p values showed that the control animals displayed a trend to perform better than the resuscitated animals.
Memory recall was impaired early after 8 min of ventricular fibrillation cardiac arrest and might be a more valuable tool for cognitive testing than learning recall after global ischemia due to cardiac arrest.
通过探索行为和认知功能，可以增强对啮齿动物模型心脏骤停后潜在保护性治疗的有益效果评估。莫里斯水迷宫是一种用于测试空间学习和记忆的著名认知范式。
对在室颤性心脏骤停8分钟后复苏的斯普拉格-道利大鼠（300±25克），在心脏骤停（CA）后5周和12周进行莫里斯水迷宫行为测试，并与未处理大鼠（对照组）的结果进行比较。在5周时，每组内到达隐藏平台的潜伏期时间（反映空间学习）从第1天到第4天同样减少（CA组：105.6±8.2对8.9±1.2秒，p<0.001；对照组：75.5±13.2对17.1±4.5，p<0.001），组间无差异（p=0.138）。在最后一次试验后24小时的探索试验中，每组内在目标区域停留的时间（反映记忆回忆）显著长于其他三个区域中的任何一个（CA组：25±1.3；对照组：24.7±2.5秒），但组间无显著差异。7天后（反映记忆保持），与其他任何区域相比，对照组动物在目标区域停留的时间仍显著更长，而心脏骤停组动物则不然。即使在心脏骤停12周后，单个p值显示对照组动物表现出比复苏动物更好的趋势。
室颤性心脏骤停8分钟后早期记忆回忆受损，对于认知测试而言，它可能是比心脏骤停导致的全脑缺血后的学习回忆更有价值的工具。
