Moats W A, Maddox S E
Can J Microbiol. 1978 Jun;24(6):658-61. doi: 10.1139/m78-110.
Four common dyes were tested as inhibitors of four types of bacteria over the pH range 5.0-9.0. Inhibition of the gram-negative types, Salmonella anatum and Enterobacter aerogenes, was markedly affected by the pH of the medium. These organisms tolerated concentrations of crystal violet and ethyl violet about 100-fold higher at pH 5.0 than at pH 9.0. Above pH 7.0 brilliant green (BG) and malachite green (MG) were precipitated as their respective carbinols and lost their inhibitory properties with these two organisms. Two gram-positive types, Staphylococcus aureus and Bacillus cereus, were more sensitive to dyes and results were less affected by pH. The carbinol forms of MG and BG were nearly as inhibitory to these organisms as the ionized forms.
在pH值5.0 - 9.0范围内，测试了四种常见染料作为四种细菌类型的抑制剂。革兰氏阴性菌，即鸭沙门氏菌和产气肠杆菌，其抑制作用受到培养基pH值的显著影响。在pH 5.0时，这些微生物对结晶紫和乙基紫的耐受浓度比在pH 9.0时高约100倍。在pH 7.0以上，亮绿（BG）和孔雀绿（MG）会沉淀为各自的醇形式，并对这两种微生物失去抑制特性。两种革兰氏阳性菌，即金黄色葡萄球菌和蜡样芽孢杆菌，对染料更敏感，结果受pH值影响较小。MG和BG的醇形式对这些微生物的抑制作用几乎与离子化形式相同。
